同济大学学报（医学版）2011，Vol.32Issue(1)：5-10,6.DOI:10.3969/j.issn1008-0392.2011.01.005
SOCS1与经典型慢性骨髓增殖性肿瘤关系的研究
Study of SOCS1 gene in typical myeloproliferative neoplasms
摘要
Abstract
Objective To study the function and mechanism of signal transduction of signaling ( SOCS) in typical myeloproliferative neoplasms( MPNs) patients.Methods By using DNA sequencing detection SOCS1 mutation and methylation specific PCR detection SOCS1 methylation, the real-time PCR was emploied to check SOCS1 expression.Results Methylation of SOCSI was detected in 37 out of 100(37％ ) MPNs: 15 out of 44 ( 34％ ) PV, 19 out of 48 (38％ ) ET,3 out of 8(37.5％ ) MF;Methylation of SOCS1 detected in control groups was 21 out of 173 ( 12％ ) : 15 out of 93 ( 16％ ) CML,4 out of 30(13.3％ ) MDS, 2 out of 30(6.7％ ) AML, 0 out of 20 healthy wolunteers.Methylation of SOCS1 greatly inhibited gene expression in MPNs as compared with no SOCS methylation ( P ＜ 0.05 ) .The presence of SOCS1 methylation was found to be significantly at advanced age at the time of diagnosis and with higher leukocyte count than patients with unmethylated.SOCS1 methylation patients were more prone to progress into typical MPN as compared with non - SOCSI methylation patients.Nonmethylation patients were not associated with peripheral blood counts and age.No mutation was found in gene sequencing of SOCS1.Conclusion Methylation of SOCS1 exists in MPNs which inhibits SOCS1 gene expression and the development and metastasis.SOCS1 methylation is related to advanced age,higher leukocyte counts and hemoglobin level in MPNs.SOCS1 hyper-methylation can activate JAK -STAT signaling pathways or accompany JAK2 mutations.These changes may represent a potential therapeutic direction.关键词
骨髓增殖性疾病/细胞因子信号转导蛋白抑制因子1/RT-PCR/甲基化/PCR分类
医药卫生引用本文复制引用
秦伟,陆惠娜,黄滨滨,修冰,薄兰君,傅建非..SOCS1与经典型慢性骨髓增殖性肿瘤关系的研究[J].同济大学学报（医学版）,2011,32(1):5-10,6.基金项目
国家自然科学基金资助项目(30871080,30572161) （30871080,30572161）
教育部新世纪优秀人才(81070430) （81070430）
