中国病理生理杂志2011，Vol.27Issue(1)：153-160,8.DOI:10.3969/j.issn.1000-4718.2011.01.030
定量蛋白质组学技术构建系统性红斑狼疮疾病的蛋白质组差异表达图谱
Differential expression profiling of proteome for systemic lupus erythematosus with quantitative proteomic technique
摘要
Abstract
AIM : To identify and quantify the total proteins in peripheral hlood mononuclear cells ( PBMC ) of systemic lupus erythematosus ( SLE ) patients with quantitative proteomic technique, and to establish a differential expression profile of proteome for SLE.METHODS: Four - plex isobaric tags for relative and absolute quantification coupled with multiple chromatographic fractionation and tandem mass spectrometry were used to analyze the total proteins in PBMC from healthy controls and the patients of stable SLE, active SLE and theumatoid arthritis.The proteins were identified by datahase searching with peptide mass fingerprinting.The differential expression of the proteins was compared.RESULTS :More than 400 proteins were identified.Compared with healthy controls , 44 proteins were discovered to be significantly expressed hy more than 2 folds in stahle SLE and active SLE, among which 9 proteins were up - regulated and 35 proteins were down - regulated.Compared with theumatoid arthritis group, 52 proteins displayed 2 or more folds of changes in stable SLE and active SLE, including 19 up - regulated proteins and 33 down - regulated ones.The up - and down - regulated proteins between active SLE and stable SLE were 17 and 13 , respectively.CONCLUSION : Quantitative proteomic technique is efficiently applicable for protein identification and relative quantitation in human peripheral blood mononuclear cells.Determination of the differentially expressed proteomic profile of SLE is helpful for better understanding the pathogenesis of SLE and developing new strategies for diagnosis and treatment of SLE.关键词
定量蛋白质组学/红斑狼疮/系统性/单个核细胞分类
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王林纤,戴勇,文锦丽,涂植光,张丽,齐素文..定量蛋白质组学技术构建系统性红斑狼疮疾病的蛋白质组差异表达图谱[J].中国病理生理杂志,2011,27(1):153-160,8.基金项目
深圳市科技计划重点资助项目(No.201001001) （No.201001001）
