昆明医学院学报2011，Vol.32Issue(1)：26-29,4.
成年恒河猴大脑皮质BDNF分离、鉴定及其分布
Isolation and Identification of BDNF in Cortex of Adult Rhesus Monkey and Its Distribution
摘要
Abstract
Objective To isolate and identify brain derived neurotrophic factor (BDNF) in the cerebral cortex of adult Rhesus monkey, and investigate its histological distribution. Methods The cortex tissues of monkeys were harvested, homogenized and isolated by SDS PAGE electrophoresis, and identified by western blot using rabbit BDNF specific antibody. Then the frozen sections from cortex were prepared, and the immunohistoebemistry method was used to detect the distribution of BDNF in the cerebral cortex of adult Rhesus monkey. The biological function of BDNF was discussed. Results After electrophoresis, Western blot showed that a specific band for BDNF located in 14KD molecule weight was recognized from the homogenization of monkey cortex. The BDNF immunopositive reaction was seen in the plasma and neurite of neurons in the layer Ⅲ and Ⅴ cerebral cortex of monkey. Conclusions Electrophoresis can isolate many proteins from the cerebral cortex of Rhesus monkey. BDNF can be recognized and indenfified by Western blot. BDNF is localized in the neurons of the cerebral cortex of monkey, suggestsing that BDNF may be a crucial molecule in the functional maintainence of CNS in adult monkeys.关键词
恒河猴/大脑皮质/Western blot/BDNF/组织分布Key words
Rhesus monkey/ Cerebral cortex/ Western blot/ BDNF/ Histological distribution分类
医药卫生引用本文复制引用
李玉,王波,王廷华,习杨彦彬..成年恒河猴大脑皮质BDNF分离、鉴定及其分布[J].昆明医学院学报,2011,32(1):26-29,4.基金项目
美国纽约中华医学基金资助项目(CMB-OO-72) （CMB-OO-72）
