中国病理生理杂志2011，Vol.27Issue(1)：1-8,8.DOI:10.3969/j.issn.1000-4718.2011.01.001
副流感病毒感染豚鼠咳嗽反射敏感性变化及其神经源性炎症机制探讨
The changes of cough reflex sensitivity in guinea pigs with parainfluenza virus infection and its neurogenic mechanism
摘要
Abstract
AIM : To investigate the changes of cough reflex sensitivity ( CRS ) in guinea pigs of human parainfluenza virus type 3( PIV3 ) infection and the mechanisms, as well as the role of neurogenic inflammation in CRS change and the pathogenesis of viral cough.METHODS: Fifty guinea pigs were randomly divided into normal control group and four virus infection groups of post - infection day ( PID ) 6 , 12,28 and 42.PIV3 was inoculated by intranasal instillation.The Buxco system was used to assess cough reflex sensitivity ( CRS ).PIV3 antigen and nucleic acid were detected by immunofluorescence and fluorescence quantitative PCR ( FQ - PCR ).Substance P ( SP ) content in lung homogenates was assayed by ELISA.NK1 receptor of SP and vanilloid receptor suhtype 1 ( VR1 ) mRNA levels in lung tissue were determined by FQ - PCR.The protein expression of lung SP, VR1 and protein gene product ( PGP - 9.5 ) were examined by immunohistochemistry and semi - quantitative analysis.The correlation hetween neuropeptides and CRS was analysed.RESULTS :The CRS of infected guinea pigs was significantly higher than that in control group, with the highest in PID 42.The viral antigen expression can he seen in hoth PIV3 - infected culture cells and lung tissues.The content of PIV3 RNA peaked in PID 6 and showed a decreasing trend with infection time.The SP and VRI mRNA in all infected groups and NK1 mRNA levels in groups of PID 12.28 and 42 increased significantly.The protein expression of SP in PID 6,12 and 28, VR1 in PID 28 and 42 and PGP - 9.5 in PID 42 enhanced obviously.Correlation analysis showed that the content of SP in PID 6and 28 , NK1 mRNA in PID 12, 28 and 42 and VR1 mRNA in all infected groups had a positive correlation with CRS.The protein expression of SP in PID 6,12 and 42, VR1 in PID 12 and PGP9 in PID 12 showed a positive correlation with CRS.CONCLUSION : The increase of neuropeptides releasing in PIV3 - infected groups suggested that neurogenic inflammation was caused.The temporal and spatial variation of CRS and neuropeptides and the positive correlation between both indicated that airway neurogenic inflammation may play a decisive role in the increase of CRS and postinfectious cough caused by viral infection.关键词
咳嗽/咳嗽反射敏感性/神经源性炎症/副流感病毒/P 物质分类
医药卫生引用本文复制引用
叶新民,钟南山,刘春丽,赖克方,陈如冲..副流感病毒感染豚鼠咳嗽反射敏感性变化及其神经源性炎症机制探讨[J].中国病理生理杂志,2011,27(1):1-8,8.基金项目
国家自然科学基金资助项目(No.30700341) （No.30700341）
