中国病理生理杂志2011，Vol.27Issue(1)：37-41,5.DOI:10.3969/j.issn.1000-4718.2011.01.007
E1A激活基因阻遏子促进人血管内皮细胞VEGF分泌和单层通透性增加
Effects of cellular repressor of E1A-stimulated genes on VEGF release and monolayer permeability of human vascular endothelial cells
摘要
Abstract
AIM : To study the effects and mechanism of cellular repressor of E1A - stimulated genes ( CREG )on VEGF release and monolayer permeability of human vascular endothelial cells ( ECs ).METHODS: The monolayer permeability of ECs was measured by transwell chamber model.The expression and localization of F - actin and VE - cadherin were examined by immunofluroscence using Olympus IX - 70 fluorescent microscope.Enzyme - linked immunosorbent assay ( ELISA ) were performed to determine the concentration of vascular endothelial growth factors( VEGF ) in the culture medium.VEGF neutrilization antibody was used to block the expression of VEGF in the cells.RESULTS : The monolayer permeability of CREG over - expressing ECs ( EO group ) was significantly higher than that of the normal control ECs ( EN group, P ＜ 0.05 ).The monolayer permeability of CREG suppressing ECs ( ES group ) was lower than that in EN group ( P ＜ 0.05 ).F - actin cytoskeleton in EO group showed disorganized, polymerized and hundled obviously to form large quantity of stress fibers in the central portion of the cells, whereas F - actin in EO group was mainly observed in the peripheral portion of the cells and only small amounts in the central portion of the cells.A widespread gap formation and a loss of VE - cadherin staining at the periphery were found in the cells of EO group.Inversely, the cells in ES group showed the localization of VE - cadherin at the cell - cell contacts tightly and the formation of zipper - like structures.Compared with EN group, the secretion of VEGF in the cell culture supernatants increased in EO group, but decreased in ES group ( P ＜0.05 ).Furthermore, the changes of ECs permeability, cytoskeleton reorganization and loss of VE - cadherin inducecl by CREG were abolished by the addition of anti - VEGF neutralizing antibody.CONCLUSION : CREG over - expression increases the monolayer permeability of ECs, induces the cytoskeleton reorganization and reduces VE - cadherin expression by enhancing the secretion of VEGF in vitro.关键词
E1A 激活基因阻遏子/血管内皮细胞/VE-cadherin/细胞骨架/通透性/血管内皮生长因子分类
医药卫生引用本文复制引用
王占胜,韩雅玲,康建,张效林,陶杰,田孝祥,闫承慧..E1A激活基因阻遏子促进人血管内皮细胞VEGF分泌和单层通透性增加[J].中国病理生理杂志,2011,27(1):37-41,5.基金项目
国家自然科学基金资助项目(No.30770793 （）
No.30971218 （）
No.30800465) （）
