中国病理生理杂志2011，Vol.27Issue(1)：67-71,5.DOI:10.3969/j.issn.1000-4718.2011.01.013
P2Y1受体介导Aβ25-35所致大鼠星形胶质细胞活化
Role of P2Y1 receptor in activation of astrocytes induced by Aβ25-35
摘要
Abstract
AIM: To study the role of P2Y1 receptor in the activation of astrocytes induced by Aβ25-35.METHODS: Astrocytes were isolated and cultured from newborn Wistar rats and divided into control group, Aβ25-35group , MRS2179( P2Y1 receptor inhibitor ) + Aβ25 -35 group and MRS2179 group by treating the cells with the corresponding reagents.The expression levels of GFAP and P2Y1 were determined by the methods of immunohistochemistry, immunofluorescence and Western hlotting.RESULTS: No significant change of the astrocyte numbers in all groups was ohserved.Compared with the control cells, the fluorescence intensity of GFAP significantly increased in Aβ25 -35 group and decreased in both MRS2179 + Aβ25 -35 group and MRS2179 group.The expression level of GFAP determined by Western blotting and immunofluorescence showed the similar trend of change in each group.Compared with control group, the expression of P2Y1 in Aβ25 -35 group was significantly increased ( P ＜ 0.05 ), and no significant change between MRS2179 + Aβ25 -35group and MRS2179 group was found ( P ＞ 0.05 ).CONCLUSION : Aβ25 -35 activates astrocytes by activation of P2Y1 receptor.关键词
受体,P2Y1/淀粉样β蛋白/星形细胞分类
医药卫生引用本文复制引用
常利,张博爱,贾延劼,付振强,沈雷,石进峰,文全庆,赵二义..P2Y1受体介导Aβ25-35所致大鼠星形胶质细胞活化[J].中国病理生理杂志,2011,27(1):67-71,5.基金项目
国家自然科学基金资助项目(No.30770758) （No.30770758）
