中国病理生理杂志2011，Vol.27Issue(1)：22-26,5.DOI:10.3969/j.issn.1000-4718.2011.01.004
5-羟基癸酸盐对慢性低氧大鼠肺动脉Kv1.5通道表达的影响
Effects of 5-hydroxydecanoate on Kv1.5 expression in pulmonary arteries of chronic hypoxic rats
摘要
Abstract
AIM : To investigate the effect of mitochondrial ATP - sensitive potassium channel( mitoKATP ) inhibitor 5 - hydroxydecanoate ( 5 - HD ) on chronic hypoxic pulmonary artery hypertension ( CHPAH ) in rats. METHODS :Twenty - four male Sprague - Dawley rats were randomly divided into 3 groups :control group, hypoxia group and hypoxia +5 - HD group.To establish an animal model of CHPAH, the rats in hypoxia group and hypoxia +5 - HD group were placed into a hypoxic and normoharic chamber [ O2( 10.0 ±0.3 )％] for 4 weeks ( 8 h a day, 6 days a week ).The mean pulmonary arterial pressure ( mPAP ) and the ratio of right ventricle weight to left ventricle with septum weight [ RV/( LV + S ) ]were measured.Western blotting and reverse transcription - polymerase chain reaction ( RT - PCR ) were performed to analyze the protein and mRNA levels of Kv1.5 in the pulmonary artery , respectively.RESULTS : The mPAP and RV/( LV +S ) were much higher in hypoxia group than those in control group, while those in hypoxia +5 - HD group were decreased as compared to hypoxia group.The protein and mRNA levels of Kv1.5 in hypoxic group were signific:antly lower than those in control group, and this decrease was partly reversed by 5 - HD.CONCLUSION : 5 - HD may exert a protective effect on CHPAH by decreasing mPAP and RV/ ( LV + S ).Inhibiting mitoKATP and stimulating Kv1.5 expression by 5 - HD may contribute to the mechanism of this effect.关键词
5-羟基癸酸盐/Kv1.5 通道/线粒体 ATP 敏感性钾通道/大鼠分类
医药卫生引用本文复制引用
李秋,张丽萍,舒鹰,李云雷,陈成水..5-羟基癸酸盐对慢性低氧大鼠肺动脉Kv1.5通道表达的影响[J].中国病理生理杂志,2011,27(1):22-26,5.基金项目
浙江省自然科学基金资助项目(No.Z2080988) （No.Z2080988）
