中国病理生理杂志2011，Vol.27Issue(1)：48-51,4.DOI:10.3969/j.issn.1000-4718.2011.01.009
老龄大鼠心房肌细胞起搏电流的特征
Characteristics of pacemaker current in aged rat atrial myocytes
摘要
Abstract
AIM : To investigate the characteristics of pacemker current ( If ) of atrium myocytes in aged rats.METHODS : Aged SD rats ( 22 - 24 months ) were used in the study.The single atrium myocytes were isolated.If current was recorded by the technique of whole - cell patch clamp.RESULTS : The densities of If increased markedly in aged rat atrium myocytes compared with that in the cells of young rats.At the test potential of - 150 mV, the average amplitude of If was ( 382 ±23 )pA and the current density was ( 3.2 ±0.4 )pA/pF, while the average amplitude of If in control group was ( 86.9 ±8.4 )pA and the current density was ( 0.9 ±0.1 )pA/pF,P ＜ 0.01.If current was augmented at negative hyperpolarzing test potential.Time constants of activation in aged and control cells were ( 230.2 ± 14.4 )ms and ( 670.5±23.6 )ms, respectively. Furthermore, steady state curves in the elder cells was shifted to positive potentials and the voltages for half maximal activation were ( -87.2 ±2.3 )mV ( aged cells ) and ( - 104.4 ±6.3 )mV ( control cells ).In contrast, the slope of activation curves and reversed potential of If were slightly different between aged cells and control cells.CONCLUSION : The amplitude and densities of If in atrium cells of aged rats were larger than that of control rats.关键词
老龄大鼠/心房肌细胞/起搏电流分类
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林琨,蓝云锋,方舟,高进辽,王红娟,李泱..老龄大鼠心房肌细胞起搏电流的特征[J].中国病理生理杂志,2011,27(1):48-51,4.基金项目
国家自然科学基金资助项目(No.30570739) （No.30570739）
