中国病理生理杂志2011，Vol.27Issue(1)：62-66,5.DOI:10.3969/j.issn.1000-4718.2011.01.012
谷氨酸致原代培养皮层神经元损伤中自噬的激活
Autophagy is activated during glutamic acid-induced cortical neuron injury
摘要
Abstract
AIM : To investigate whether autophagy is activated during glutamic acid - induced neuron injury and the possible neuroprotective effect of 3 - methyl adenine( 3 - MA ) ( an autophagy inhibitor ).METHODS : Glutamic acid or 3 - MA was added to the medium of cultured cortical neurons.Cell viability was measured by MTT assay.The formation of autophagosome was observed under transmission electron microscope.The marker protein light chain 3( LC3 ) for autophagy was detected by immunofluorescence assay and visualized under laser confocal microscope.RESULTS : The cell viability declined during glutamic acid treatment and the autophagosomes were increased.LC3 , the marker protein of autophagy, also significantly increased.The autophagy level was lowered by 3 - MA, and cell viability was increased.CONCLUSION : The results suggest that autophagy is activated during glutamic acid treatment and inhibition of autophagy may have neuroprotective effect.The autophagy inhibitor 3 - MA may be a potential neuroprotective agent.关键词
自吞噬作用/3-甲基腺嘌呤/神经元/谷氨酸分类
医药卫生引用本文复制引用
陈兆耀,陈茂刚,卢婷婷,倪冠中,刘新峰,徐格林..谷氨酸致原代培养皮层神经元损伤中自噬的激活[J].中国病理生理杂志,2011,27(1):62-66,5.基金项目
国家自然科学基金资助项目(No.30870848) （No.30870848）
