中国中西医结合杂志2011，Vol.31Issue(3)：355-358,4.
沙棘总黄酮对血管内皮细胞保护作用及机制研究
Protection and Mechanism of Total Flavone of Hippophae Rhamnoides on Vascular Endothelial Cells
摘要
Abstract
Objective To observe the protection of total flavones of Hippophae Rhamnoides (TFH) on vascular endothelial cells (VECs). Methods Human umbilical VECs (ECV304) were used. The vascular endothelial injured cell model was prepared using hydrogen dioxide ( H2O2 ). The cell apoptosis rate and changes of mean fluorescence intensity were detected using flow cytometry (FCM). The Caspase-3 activity in VECs was detected by Western blot. Results VEC apoptosis was induced by 200 μmol/L H2O2. TFH in different concentrations (400,200, and 100 μg/mL) could significantly lower the cell apoptosis rate induced by H2 O2 respectively (all P＜0. 05),and obviously inhibit Caspase-3 activities (all P＜0. 01 ). Conclusions TFH could fight against H2O2 injured VECs apoptosis. Lowedng the Caspase-3 expression was one of its mechanisms in protecting VECs.关键词
沙棘总黄酮/血管内皮细胞/Caspase-3引用本文复制引用
程嘉艺,滕丹,李伟,孙静,康廷国..沙棘总黄酮对血管内皮细胞保护作用及机制研究[J].中国中西医结合杂志,2011,31(3):355-358,4.基金项目
辽宁省科学技术计划项目(No.2005408001) （No.2005408001）
