中国全科医学2011，Vol.14Issue(5)：497-499,502,4.
山莨菪碱对缺氧复氧损伤血管内皮细胞凋亡的影响
Effects of Anisodamine on Human Umbilical Vein Endothelial Cells' Apoptosis Induced by Hypoxia-reoxygenation
摘要
Abstract
Objective To explore the influence of anisodamine on human umbilical vein endothelial cells' ( HUVECs ) apoptosis induced by hypoxia and reoxygenation. Methods The HUVECs were divided into groups control ( group A ), hypoxia - reoxygenation 0. 9％ saline ( group B ), hypoxia - reoxygenation epinephrine ( group C ), hypoxia - reoxygenation Ani ( group D ), hypoxia -reoxygenation epinephrine plus Ani ( group E ). The cell apoptoses were detected by fluorescence flow cytometry, the gene expression of Bel - 2 by reverse transcription - polymerase chain reaction ( RT - PCR ) . Results There was significant difference in apoptotic indexes ( AI ) of HUVECs at hours 12, 24 of reoxygenation between 5 groups ( P ＜ 0. 05 ), group B were significantly different from groups C, D, E ( P ＜0. 05 ), group C not from group D ( P ＞0. 05 ), but groups C, D from group E ( P ＜0. 05 ). There was significant difference in Bcl - 2mRNA level between 5 groups at hours 12, 24 of reoxygenation ( P ＜ 0. 05 ), and Bcl - 2mRNA expression increased more significantly in group Ethan in other groups ( P ＜ 0. 05 ) . Conclusion Ani can reduce apoptosis of HUNVECs after hypoxia - reoxygenation. Ani protects the function of endothelia cells after hypoxia -reoxygenation by influencing the mRNA expression of Bcl -2.关键词
山莨菪碱/血管内皮细胞/细胞凋亡分类
医药卫生引用本文复制引用
孙菁,孟凡山,陈威,计达,沈洪..山莨菪碱对缺氧复氧损伤血管内皮细胞凋亡的影响[J].中国全科医学,2011,14(5):497-499,502,4.基金项目
国家高技术研究发展计划(863)项目(2002AA2Z3321) （863）
