中国病理生理杂志2011，Vol.27Issue(1)：129-133,5.DOI:10.3969/j.issn.1000-4718.2011.01.025
后肾细胞微环境诱导胚胎干细胞向肾系细胞分化的实验研究
Metanephric cell microenvironment induces embryonic stem cells to differentiate toward renal cells
摘要
Abstract
AIM: To explore the effects of metanephric cell microenvironment on inducing embryonic stem cells ( ESCs ) to differentiate toward renal cells.METHODS : Emhryoid bodies ( EBs ) of D3 mouse emhryonic stem cells were prepared by hanging drop culture, and the EBs were co - cultured indirectly with metanephric cells derived from E12.5 d mouse embryo.The EBs cell with spontaneous differentiation was used as the control.The proteins of Pax2 and WT - 1 were analyzed by immunofluorescence assay.The mRNA expression of Pax2.WT - 1, Lim1 , Sall1 , Emx2 , GDNF, Wnt4 , BMP7 , Nephl, Nephrin, KSP and CD24 genes was detected by RT - PCR.RESULTS: The genes related to kidney development were expressed in the EBs cells after co - culture on day 3, and the mRNA expression of Pax2,WT -1 Emx2, GDNF, Nephl, Nephrin, KSP and CD24 was stronger than those in control group.Pax2 positive cells were found on day 3 in the co - cultured EBs cells, and the positive cells increased on day 5 and day 7.WT - 1 protein positive cells were found in the co - cultured EBs cells on day 5.No Pax2 or WT - 1 positive cell was ohserved in control group.CONCLUSION : Metanephric cell microenvironment promotes ESCs differentiation toward renal cells.关键词
胚胎干细胞/后肾细胞/肾系细胞分类
医药卫生引用本文复制引用
汪泱,张立行,王共先,谢安,娄远蕾,阮琼芳,崔苏萍,杨阳..后肾细胞微环境诱导胚胎干细胞向肾系细胞分化的实验研究[J].中国病理生理杂志,2011,27(1):129-133,5.基金项目
国家自然科学基金资助项目(No.30960385) （No.30960385）
江西省自然科学基金资助项目(No. 2007GZY1470) （No. 2007GZY1470）
