中国循证儿科杂志2011，Vol.6Issue(1)：37-41,5.DOI:10.3969/j.issn.1673-5501.2011.01.006
肺表面活性物质蛋白B基因多态性与新生儿呼吸窘迫综合征易感性的研究
Relationship between polymorphisms of surfactant protein B gene and neonatal respiratory distress syndrome susceptibility
摘要
Abstract
Objective To study the relationship between gene polymorphisms of surfactant protein B ( SPB ) and neonatal respiratory distress symdrome susceptibility.Methods Children diagnosed as neonatal respiratory distress syndrome( NRDS ) from Tongji Hospital, Tongji Medical College , Huazhong University of Science and Technology were enrolled.The children born at the same gestation age were taken as control group and the ratio of NRDS group to control group was 1: 2.Genomic DNA was extracted from peripheral blood by EDTA anticoagulation, using a standard Phenol/chloroform extraction procedure.The genetic polymorphisms of SPB-I8A/C and SPB1580C/T were analyzed with polymerase chain reaction restriction fragment length polymorphism( PCR-RFLP ) using the restriction enzyme Apapl and Dde Ⅰ.Statistical analyses were carried out using SPSS software version 13.0.The Hardy-weinberg equilibrium was determined for both NRDS and control groups.X2 test was used to detect the difference between two groups.Results Ninety one children with NDRS were chosen as NRDS group and 182 children were enrolled as control group from 2008 to 2010.①The SPB-I8 AA, AC , and CC genotype frequencies in NRDS group were 11.0％,40.7％, 48.4％ versus 6.6％ , 31.3％ , and 62.1％ in control group, hoth were with no significant difference( P ＞ 0.05 ).The frequencies of A allele were higher in NRDS group than those in control group( 22.3％ v.s 31.3％ ).②The SPB1580 TT, TC , and CC genotype frequencies in NRDS group were 5.5％ , 63.7％ , 45.1％versus 30.8％ , 6.6％ , and 48.4％ in control group, both were with no significant difference( P ＞ 0.05 ).The frequencies of C allele were higher in NRDS group than those in control group ( 79.1％ v.s 70.9％ ).③ The genotype frequencies of 1580 C among Han and American.Brazilian.Dane were 79％ , 35％ , 41％ and 46％( P ＜0.05 ) and 72％ in Japanese( P ＞ 0.05 ).The Genotype frequencies of -18A among Han and American, Brazilian,Dane were 31％ , 58％ ,57％ and 61％( P＜0.05 ).Conclusion The genetic polymorphisms of SPB-I8A/C and SPB1580C/T were the independent risk factors of neonatal respiratory distress syndrome.The polymorphisms of SPB-I8A/C and 1580C/T differ among races.关键词
肺泡表面活性物质蛋白B/基因多态性/新生儿呼吸窘迫综合征/易感性引用本文复制引用
曾凌空,李文斌,潘睿,单瑞艳,周玉荣,张佳,常立文..肺表面活性物质蛋白B基因多态性与新生儿呼吸窘迫综合征易感性的研究[J].中国循证儿科杂志,2011,6(1):37-41,5.基金项目
国家自然科学基金资助项目:30872795 （）
