广东医学2011，Vol.32Issue(4)：427-429,3.
BALB/c小鼠1型糖尿病模型的构建与CD4+ CD25+Treg的检测
Establishment of type 1 diabetic BALB/c mouse models and detection of CD4+ CD25+regulatory T cells
刘繁荣 1钟清玲 2刘德伍 3彭燕 3赵彤4
作者信息
- 1. 南昌大学医学院病理教研室,南昌,330006
- 2. 南昌大学研究生学院,南昌,330006
- 3. 南昌大学第一附属医院烧伤研究所,南昌,330006
- 4. 南方医科大学基础医学院病理学系,广州,510515
- 折叠
摘要
Abstract
Objective To establish type 1 diahetic BALB/c mouse models and investigate the significance of CD4+ CD25 + regulatory T cells ( Treg ) in TIDM. Methods Sixty BALB/c mice were randomly divided into four groups ( n =15 ). BALB/c mouse diabetic models were established with single intraperitoenal ( ip ) injection of 150 mg/kg of streptozotocin ( STZ ) and 5 separated ip injection of 40 mg/kg of STZ in Group A and Group C, respectively, while controls were set up with corresponding injections of citrate buffer solution in Group B and Group D, respectively. Blood sugar level was measured. The frequency of CD4+ CD25 + Treg cells in peripheral blood was determined hy flow cytometry. Results Type Ⅰ diabetes mellitus ( TIDM ) was observed in 86. 67％ and 93. 33％ mice of Group A and Group C, respectively , with suspended high blood glucose since the 2lth day. No significant difference was revealed between Group A and C ( P ＞ 0. 05 ). The peripheral CD4 + CD25 + Treg cell proportion was significantly lower in model groups than that in control group[ ( 4. 05 ±0. 37 )％ vs ( 8. 68 ±0. 45 )％ , P ＜0. 05 ]. Conclusion Single intraperitoneal injection of STZ is an effective method in construction of BALB/c TIDM mouse.关键词
BALB/c小鼠/链脲佐菌素/糖尿病/调节性T细胞引用本文复制引用
刘繁荣,钟清玲,刘德伍,彭燕,赵彤..BALB/c小鼠1型糖尿病模型的构建与CD4+ CD25+Treg的检测[J].广东医学,2011,32(4):427-429,3.
