重庆医学2011，Vol.40Issue(7)：676-677,682,后插4,4.DOI:10.3969/j.issn.1671-8348.2011.07.021
α-生育酚缓解链脲佐菌素诱导乳鼠胰岛细胞损伤的研究
Effects of α-tocopherol on streptozotocin-induced apoptosis of neonatal rat pancreatic cells
袁英 1张艳 1宋仕卿 1郭兵1
作者信息
- 1. 重庆医药高等专科学校基础部,401331
- 折叠
摘要
Abstract
Objective To investigate the effect of atocopherol on the streptozotocin-induced damage of rat islet cells. Methods Isolated islet cells from wistar rats of 1-3 d incubated in monolayer in vitro. β-cell morphology,islet cell viability,apoptotic rate of islct cells,expression of Bcl-x1 and Bax after incubation with streptozotocin were measured,and the potential effects of α-tocopherol was investigated. Results (1) α-tocopherol increased the islet cell viability in a dose-dependent fashion. (2)Compared with the control group, apoptotic rate of pancreatic islet cells increased, Bcl-xl expression decreased and Bax gene expression increased when cocultured with streptozotocin(P＜0. 01). (3)Compared with the STZ group, apoptotic rate of pancreatic islet cells decreased.Bcl-xl expression increased and Bax gene expression decreased in the α-tocophrrol group(P＜0. 01 ). Conclusion α-tocophcrol can effectively attenuate streptozotocin-induced damage of rat islet cells by means of regulating Bcl-xl and Bax gene expressions.关键词
α生育酚/链脲菌素/胰岛素分泌细胞/bcl-X蛋白质/bcl-2相关X蛋白质引用本文复制引用
袁英,张艳,宋仕卿,郭兵..α-生育酚缓解链脲佐菌素诱导乳鼠胰岛细胞损伤的研究[J].重庆医学,2011,40(7):676-677,682,后插4,4.
