重庆医学2011，Vol.40Issue(7)：625-627,630,4.DOI:10.3969/j.issn.1671-8348.2011.07.001
食管鳞状细胞癌易感性与MMP-9 C-1562T基因多态性的关联研究
Correlation between polymorphisms of matrix metalloproteinase-9 and esophageal squamous cell carcinoma patients in southwest China
摘要
Abstract
Objective To explore the effcct of differcnt doses of artcsunate on the level of interleukin-17 and matrix metalloprotcinases-3(MMP-3) and matrix metalloproteinascs-9(MMP-9) in rat of adjuvant arthritis. Methods 80 clean grade male Wistar rats were selected to establish model adjuvant arthritis. On 6 d after modeling,60 rats with the sum of arthritis index of right meta pedes and two propodium≥6 were selected, and randomly divided into 6 groups(n = 10). Model control group was infused with normal saline[10 mL/( kg · day) ] for 7 d; the other groups, artesunate groups, were infused with different doses of artesunate[ ( 2. 5 , 5,10,20,40 mg/(kg · day)] and normal saline(2-4 mL) for 7 d. 10 normal Wistar rats were selected as controls. On 8 d after treatment, enzyme-linked immunosorbent assay( ELISA) was used to detect serum level of IL-17, MMP-3 and MMP-9. Results Compared with model rats,the serum level of IL-17 and MMP-3 and MMP-9 in artesunate groups were significantly decreased in a dose-depedent manner(P＜ 0. 01). The group[20mg/( kg · day)] was the top in artesunate groups. Conclusion Artesunate can down-regulate the serum level of IL-17,MMP-3 and MMP-9 of adjuvant arthritis in a dose-depentment manner.关键词
食管肿瘤/基质金属蛋白酶/多态性,单核苷酸/疾病易感性引用本文复制引用
傅勇,胡华梅,谭益,唐胜军,熊刚,杨康,白云..食管鳞状细胞癌易感性与MMP-9 C-1562T基因多态性的关联研究[J].重庆医学,2011,40(7):625-627,630,4.基金项目
国家自然科学基金资助项目(30872549) （30872549）
重庆市自然科学基金资助项目(2009BA5013). （2009BA5013）
