重庆医学2011，Vol.40Issue(7)：628-630,后插1,4.DOI:10.3969/j.issn.1671-8348.2011.07.002
青蒿琥酯对佐剂性关节炎大鼠血IL-17、MMP-3及MMP-9的影响
Influence of artesunate on level of interleukin-17,matrix metalloproteinases-3 and matrix metalloproteinases-9 in rat of adjuvant arthritis
摘要
Abstract
Objective To explore the effcct of differcnt doses of artcsunate on the level of interleukin-17 and matrix metalloprotcinases-3(MMP-3) and matrix metalloproteinascs-9(MMP-9) in rat of adjuvant arthritis. Methods 80 clean grade male Wistar rats were selected to establish model adjuvant arthritis. On 6 d after modeling,60 rats with the sum of arthritis index of right meta pedes and two propodium≥6 were selected, and randomly divided into 6 groups(n = 10). Model control group was infused with normal saline[10 mL/( kg · day) ] for 7 d; the other groups, artesunate groups, were infused with different doses of artesunate[ ( 2. 5 , 5,10,20,40 mg/(kg · day)] and normal saline(2-4 mL) for 7 d. 10 normal Wistar rats were selected as controls. On 8 d after treatment, enzyme-linked immunosorbent assay( ELISA) was used to detect serum level of IL-17, MMP-3 and MMP-9. Results Compared with model rats,the serum level of IL-17 and MMP-3 and MMP-9 in artesunate groups were significantly decreased in a dose-depedent manner(P＜ 0. 01). The group[20mg/( kg · day)] was the top in artesunate groups. Conclusion Artesunate can down-regulate the serum level of IL-17,MMP-3 and MMP-9 of adjuvant arthritis in a dose-depentment manner.关键词
青蒿素/关节炎,实验性/白细胞介素/基质金属蛋白酶引用本文复制引用
莫汉有,王丽芳,周润华,杨敏,许佳,李宝贞,石宇红..青蒿琥酯对佐剂性关节炎大鼠血IL-17、MMP-3及MMP-9的影响[J].重庆医学,2011,40(7):628-630,后插1,4.基金项目
广西卫生厅课题资助项目(Z2006204,Z2009044) （Z2006204,Z2009044）
广西桂林市攻关课题资助项目(20090538). （20090538）
