肿瘤预防与治疗2011，Vol.24Issue(1)：9-13,5.DOI:10.3969/j.issn.1674-0904.2011.01.003
大鼠急性放射性肝损伤及其保护的实验研究
Study on Mechanisms of Radiation-induced Hepatic Injury and Its Protection
夏红强 1邓琳 1金冶宁1
作者信息
- 1. 上海交通大学医学院附属瑞金医院,肿瘤放化疗科,上海,200025
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摘要
Abstract
Objective : To observe the effects of oxygen free radical( ROS )、nitric oxide( NO )and TGF-β1 on the acute radiation-induced hepatic injury of rats. The protective effect of amifostine against radiation was also discussed. Methods: Forty-eight SDrats were divided into normal group, radiated group, and amifostine-pretreated group. The models were made hy a single radiation of 20Gy on whole liver except the normal group. Amifostine was given intraperitoneally ( 200mg/kg ) 30 minutes before irradiation to amifostine group. The levels of plasma ROS、NO、 TGF-β1 in rats were measured on the 4th，8th and 14th day after radiation. Animals were euthanized 14 days after irradiation， and the hepatic tissues were observed under microscope and electromicroscope. The plasma concentrations of AST、ALT of rats were also measured. Results : The hepatic cells showed no difference among three group under the microscope, but some changes were observed by the transmission electromicroscope. No significant difference of AST、ALT were observed among three groups. The concentrations of ROS significantly increased in radiated group ( P ＜ 0. 05 ). In group Amifostine , the activity of SOD increased while the level of MDA decreased compared with the normal group( P ＜ 0. 05 ). The concentrations of NO were significantly higher in radiated group( P < 0. 05 ). On the 14th day after radiation the concentrations of TGF-β1 increased significantly in radiated group ( P＜O. 05 ). Conclusion: ROS.NO and TGF-β1 play an important part in the acute radiation-induced hepatic injury of rats. Amifostine has a marked protective effect to hepatic injury. The plasma concentration changes of ROS、NO、TGF-β1 are much earlier than AST、ALT of rats with radiation-induced hepatic injury.关键词
放射性肝损伤/活性氧自由基/一氧化氮/TGF-β1/安福定分类
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夏红强,邓琳,金冶宁..大鼠急性放射性肝损伤及其保护的实验研究[J].肿瘤预防与治疗,2011,24(1):9-13,5.
