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番红花EST资源的SSR信息分析

陈国庆

广西植物2011,Vol.31Issue(1):43-46,96,5.
广西植物2011,Vol.31Issue(1):43-46,96,5.DOI:10.3969/j.issn.1000-3142.2011.01.010

番红花EST资源的SSR信息分析

Analysis of SSR information in EST resource of saffron crocus (Crocus sativus)

陈国庆1

作者信息

  • 1. 湖北师范学院,生命科学学院,湖北,黄石,435002
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摘要

Abstract

6745 ESTs of saffron crocus in the public database of NCBI were downloaded and analysed, resulting in 1431 non-redundant ESTs with total length about 612.01 kb. A total of 108 SSRs distributed in 103 ESTs were detected,accouting for 7.55% of the non-redundant ESTs. The average distribution distance of these EST-SSRs was about 5.67 kb. Dinucleotide and trinucleotide repeats were the main types in Crocus sativus, accouting for 30.56% and 37. 96% of all the SSRs,respectively. AG/CT and AAG/CTT are the most frequent motifs,accounting for 66. 67%and 29. 27 % in dinucleotide and trinucleotide repeats, respectively. The present study provides a base for the development and further applification of EST-SSR markers in saffron crocus.

关键词

番红花/药用植物/EST/EST-SSR/微卫星

Key words

saffron crocus/ medicinal plants/ EST/ EST-SSR/ microsatellite

分类

生物科学

引用本文复制引用

陈国庆..番红花EST资源的SSR信息分析[J].广西植物,2011,31(1):43-46,96,5.

基金项目

国家自然科学基金(30900157) (30900157)

湖北省教育厅青年项目(Q20082201) (Q20082201)

湖北师范学院人才引进项目(2007F13) (2007F13)

广西植物

OA北大核心CSCDCSTPCD

1000-3142

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