广西植物2011,Vol.31Issue(1):43-46,96,5.DOI:10.3969/j.issn.1000-3142.2011.01.010
番红花EST资源的SSR信息分析
Analysis of SSR information in EST resource of saffron crocus (Crocus sativus)
摘要
Abstract
6745 ESTs of saffron crocus in the public database of NCBI were downloaded and analysed, resulting in 1431 non-redundant ESTs with total length about 612.01 kb. A total of 108 SSRs distributed in 103 ESTs were detected,accouting for 7.55% of the non-redundant ESTs. The average distribution distance of these EST-SSRs was about 5.67 kb. Dinucleotide and trinucleotide repeats were the main types in Crocus sativus, accouting for 30.56% and 37. 96% of all the SSRs,respectively. AG/CT and AAG/CTT are the most frequent motifs,accounting for 66. 67%and 29. 27 % in dinucleotide and trinucleotide repeats, respectively. The present study provides a base for the development and further applification of EST-SSR markers in saffron crocus.关键词
番红花/药用植物/EST/EST-SSR/微卫星Key words
saffron crocus/ medicinal plants/ EST/ EST-SSR/ microsatellite分类
生物科学引用本文复制引用
陈国庆..番红花EST资源的SSR信息分析[J].广西植物,2011,31(1):43-46,96,5.基金项目
国家自然科学基金(30900157) (30900157)
湖北省教育厅青年项目(Q20082201) (Q20082201)
湖北师范学院人才引进项目(2007F13) (2007F13)