肿瘤预防与治疗2011，Vol.24Issue(1)：4-8,5.DOI:10.3969/j.issn.1674-0904.2011.01.002
线粒体细胞色素氧化酶亚单位I表达载体的构建
Construction of Pmito-RFP-CO I Recombinant Vector
摘要
Abstract
Objective : Pmito-RFP-CO Ⅰ recombinant vector was constructed and transfected into p0SK-Hepl. Pmito-RFP-CO Ⅰ expression and biological behavior of transfected pOSK-Hep1 cells were assessed. Methods : Pmito-RFP-COX Ⅰ recombinant vector was constructed by molecular cloning and transfected into p0SK-Hep1 cells. The growth status of transfected p0SK-Hep1 cells was determined hy MTT, and expression of CO Ⅰ was assessed by Western blotting. Results: The p0SK-Hep1 cells transfected with pmito RFP-CO Ⅰ recombinant vector was found to grow faster than that of the parent cells but slower than that of p0SK-Hep1 c:ells( P ＜0. 01 ). CO Ⅰ expression was found in those transfected cells. Conclusion: The function of mitochondria may be recovered partially in the p0 SK-Hep1 cells transfected with pmito-RFP-CO Ⅰ .关键词
重组载体Pmito-RFP-CO I/构建/线粒体的功能分类
医药卫生引用本文复制引用
张国桥,陈宏,刘跃,凌贤龙..线粒体细胞色素氧化酶亚单位I表达载体的构建[J].肿瘤预防与治疗,2011,24(1):4-8,5.基金项目
国家自然科学基金(30470865) （30470865）
