广西植物2011，Vol.31Issue(1)：36-38,42,4.DOI:10.3969/j.issn.1000-3142.2011.01.008
盐肤木基因组DNA提取方法改进及AFLP体系的建立
A modified method of genomic DNA extraction and establishment of AFLP systems in Rhus chinensis
摘要
Abstract
A modified method(4 × CTAB)was used to extract the effective genomic DNA from the leaves of Rhus chinensis ,and the systems of DNA restriction reaction,ligase reaction,pre-amplification and selective amplification reaction were established in this study. Using the two kinds of primer-combined methods (E+3/M+3 and E+2/M+3),8 pairs of primers were finally selected to amplify the genomic DNA ofR. chinensis,and dear bands with high genetic polymorphism and difference were obtained. The present results provided a strong foundation for the further examination of population genetic diversity and structure of R. chinensis using AFLP method.关键词
盐肤木/DNA提取/4×CTAB法/AFLP技术Key words
Rhus chinensis ； DNA extraction/ 4 × CTAB method； AFLP technology分类
农业科技引用本文复制引用
庞雅文,段立柱,宋德应,任竹梅..盐肤木基因组DNA提取方法改进及AFLP体系的建立[J].广西植物,2011,31(1):36-38,42,4.基金项目
国家自然科学基金(30670361) （30670361）
山西省自然科学基金(2007011078) （2007011078）
