广西植物2011，Vol.31Issue(1)：39-42,4.DOI:10.3969/j.issn.1000-3142.2011.01.009
厚壳桂新微卫星体分子标记的开发与应用
Isolation and characterization of new set of microsatellite loci in Cryptocarya chinensis
摘要
Abstract
Habitat fragmentation is the main threat to global biodiversity. Common species could also greatly suffer from habitat fragmentation. As a result of the destruction of the monsoon evergreen broad-leaved forest in South China,Cryptocarya chinensis ,one of the climax species,is now patchily distributed. Because a few microsateilite loci reported previously might cause low resolution to detect asexual reproduction in this species,we reported a new set of microsatellite which will assist to detect the extensiveness of sexual and asexual reproduction at fine spatial scale in the future. Eleven microsatellite markers from repetitive NA enriched libraries for C. chinensis were developed.Twenty-one individuals from Dinghu Mountain in South China were used to characterize their polymorphism. The number of alleles of 11 loci ranged from two to three,observed end expected heterozygosities ranged from 0. 048 to 1. 000,and 0. 048 to 0. 535,respectively. Deviations from Hardy-Weinberg equilibrium were detected in 8 loci due to heterozygote excess (highly significant negative Fis). All locus pairs showed significant linkage disequilibrium at the 0. 05 significant level, but such significance disappeared alter Bonferroni correction. 20 of 21 detected individuals showed identical multilocus genotype. All these results conformed to the genetic characteristic of clonal species,confirming extensive clonal growth in C. chinensis.关键词
无性生殖/保育/森林片断化/遗传标记Key words
asexual reproduction/ conservation/ forest fragmentation/ genetic marker分类
农业科技引用本文复制引用
高丹丹,王峥峰,朱鹏,叶万辉..厚壳桂新微卫星体分子标记的开发与应用[J].广西植物,2011,31(1):39-42,4.基金项目
国家重点基础研究"973"发展计划项目(2007CB411600) （2007CB411600）
国家科技支撑计划项目(2008BAC39B02) （2008BAC39B02）
中国科学院知识创新工程重大项目(KZCX2-YW-430) （KZCX2-YW-430）
"十一五"国家科技支撑计划项目(2008BAD0B05)[Supported by National Basic Research"973"Program of China(2007CB411600) （2008BAD0B05）
the National Key Technology R & D Program(2008BAC39B02) （2008BAC39B02）
Knowledge Innovation Project of the Chinese Academy of Sciences(KZCX2-YW-430) （KZCX2-YW-430）
the"Eleventh Five-Year"National Science and Technology Supporting Project(2008BAD0B05)] （2008BAD0B05）
