湖南中医药大学学报2011，Vol.31Issue(2)：3-5,16,4.DOI:10.3969/j.issn.1674-070X.2011.02.001.003.04
ephrinB2真核表达载体的构建及其在Hela细胞的表达
Construction and in Vitro Expression of an Eukaryotic Vector Encoding ephrinB2
摘要
Abstract
Objective To construct an eukaryotic plasmid expressing ephrinB2 and test whether the plasmid can be expressed in the eukaryotic cells in vitro for study the effect of ephrinB2 on tumor growth and angiogenesis.Methods The full-length ephrinB2 cDNA fragment were amplified by RT-PCR.Then the PCR product was inserted into eukaryotic fluorescent expressing vector p EGFPN1 by DNA recombinant technology and further transfected into HeLa cervical carcinoma cells.Expression of ephrinB2 in transfectants was examined by the fluorescence microscope,RT-PCR and Western blot.Results The 1kbp DNA fragment of ephrinB2 was correctly amplified.The recombinant pEGFPN1-ephrinB2 was successfully constructed and could be correctly expressed in the Hela cells.Conclusion This recombinant plasmid pEGFPN1-ephrinB2 and the resultant Hela cell model provide a basis for further study on the function of ephrinB2.关键词
ephrinB2/载体构建/蛋白表达分类
医药卫生引用本文复制引用
谢秋群,唐雄志,王世宣..ephrinB2真核表达载体的构建及其在Hela细胞的表达[J].湖南中医药大学学报,2011,31(2):3-5,16,4.基金项目
桂林市科技科技成果转化与应用项目(20080320-4). （20080320-4）
