生态学杂志2011，Vol.30Issue(1)：87-92,6.
RISA、DGGE和2D-PAGE技术对大豆根际土壤细菌多样性分析的比较
Bacterial diversity in rhizosphere soil of soybean: A comparison of RISA, DGGE, and 2D-PAGE techniques
摘要
Abstract
The PCR-based RISA, DGGE, and 2D-PAGE were adopted to analyze the bacterial diversity in thizosphere soil of soybean. Most abundant soil bacterial diversity was acquired by 2D-PAGE (gene spots) , followed by DGGE ( gene bands) , and by RISA ( gene bands). The RISA gave the fewest bands. but the result was more stable and the experimental operation was more easy; DGGE obtained more bands and showed good precision, but had more error sources; while 2D-PAGE as a novel soil microbial research method could overcome the low-resolution problem of the other two methods, achieve abundant information of soil bacterial diversity, and play an important role in the research of soil microbial ecology. However, the operation of 2DPAGE was more complicated and required strict conditions, compared with RISA and DGGE. In spite of these disadvantages, 2D-PAGE had shown potential advantages in the research field of soil microbial ecology.关键词
RISA/DGGE/2D-PAGE/细菌多样性/土壤微生物分类
农业科学引用本文复制引用
李刚,赵建宁,文都日乐,杨殿林..RISA、DGGE和2D-PAGE技术对大豆根际土壤细菌多样性分析的比较[J].生态学杂志,2011,30(1):87-92,6.基金项目
转基因生物新品种培育重大专项(2008ZX08012-04)和中央级公益性科研院所基本科研业务费专项资金(2008-LG-04)资助. （2008ZX08012-04）
