生态学杂志2011，Vol.30Issue(1)：1-6,6.
背角无齿蚌基因组(GT)n微卫星DNA特征
Characterization of (GT)n microsatellite in Anodonta woodiana genome
摘要
Abstract
The method of magnetic-bead hybridization enrichment was used to isolate the microsatellite loci of Anodonta woodiana. Genomic DNA was digested with restriction enzyme Sau3AI. The fragments were hybridized with biotin-labeled ( GT)15 probe and captured by streptavidincoated magnetic beads. The target fragments were inserted to PGEM-T vectors, and positive clones were obtained through PCR screening the library. By isolating the ( GT) microsatellite in A. woodiana genome, 180 positive clones were sequenced, 37 sequences contained microsatellite having at least 5 times repeats numbers ( 20. 6％ of white colonies screened) . From the microsatellites sequence, the two bases repeat arrays were most abundant, accounting for 86. 4％ , among which, 32. 0％ were perfect repeats, 10. 0％ imperfect repeats％ 8. 0％ compound repeats, and 50. 0％ the combination of imperfect repeats and compound repeats. The largest repeat number of perfect type microsatellite was 65 . This allowed us to design 30 pairs of primers from 37 microsatellite sequences. As a result, ll pairs were screened and used successfully to amplify polymorphic fragments.关键词
背角无齿蚌/磁珠富集法/微卫星分类
生物科学引用本文复制引用
汪桂玲,苏翔,李家乐,白志毅..背角无齿蚌基因组(GT)n微卫星DNA特征[J].生态学杂志,2011,30(1):1-6,6.基金项目
国家自然科学基金项目(30871923),上海市科委地方院校能力建设项目(08390510100)和上海市教育发展基金会晨光计划项目(2008CG56)资助. （30871923）
