安徽医科大学学报2011，Vol.46Issue(4)：355-357,399,4.
不同超排方法对家兔超数排卵效果的影响
Effect of different treatment methods on superovulation of rabbits
王田娟 1章志国 1邢琼 1周平 1曹云霞1
作者信息
- 1. 安徽医科大学第一附属医院生殖中心,合肥,230022
- 折叠
摘要
Abstract
Objective To explore an stable and high efficient superovulation method for rabbits. obtain sufficient high-quality rabbit oocytes, and provid reliable materials for the following relevant test. Methods 30 New Zealand white rabbits randomly divided into 3 groups. As for HMG + HCG group , the female rabbit was subcutaneous injected on its neck for 2 times at intervals of 24 h ; and for PMSG + HCG test group, the female rabbit was subcutaneous injected on its neck with PMSG 80 IU/pc ; and for FSH + HCG test group , the female rabbit was injected on its neck for 6 times at intervals of 12 h . and total dose was 60 IU/pc. After HMG .PMSG and FSH injection , the intravenous injection through the ear edge vein with HCG 100 IU per rabbit at the given time , the egg recovery rate was calculated, and the superovulation effect of three treatment methods was compared. Results The average number of follicles recovered,hyperaemia follicles on the ovary and ovarian cysts of the three groups had significant differences.The average number of follicles recovered from HMG +HCG group was significantly higher than those from FSH＋HCG group and PMSG +HCG group ( P ＜0. 05 ). And the average number of follicles recovered form FSH ＋ HCG group was significantly higher than that from PMSG + HCG group ( P ＜ 0. 05 ). And we found that the average number of hyperaemia follicles and ovarian cysts on the ovary of PMSG +HCG group was significantly higher than those from HMG ＋ HCG group and FSH + HCG group ( P＜ 0. 05 ). A part of the oocytes had abnormal morphology( such as first polar body broken, egg week gap, cytoplasm abnormal morphology ) which was recovered form PMSG ＋HCG group. Conclusion The most efficient superovulation methods is HMg + HCG group on rabbits.关键词
家兔/超排卵/药物作用/尿促卵泡素/绒毛膜促性腺激素/卵泡刺激素分类
医药卫生引用本文复制引用
王田娟,章志国,邢琼,周平,曹云霞..不同超排方法对家兔超数排卵效果的影响[J].安徽医科大学学报,2011,46(4):355-357,399,4.
