安徽医科大学学报2011，Vol.46Issue(4)：332-336,5.
E2F-1在胃腺癌细胞内质网应激反应中对Mcl-1的调控作用
Regulation of Mcl-1 by E2F-1 in endoplasmic reticulum stress in gastric adenocarcinoma cells
摘要
Abstract
Objective To explore the role of Mcl-1 in resistance of gastric adenocarcinoma cells to endoplasmic reticulum ( ER ) stress-induced apoptosis and the mechanism of regulation of Mcl-1 by E2F-1 in the cells under ER stress. Methods Apoptosis was measured by flow cytometry using propidium iodide staining. Western blot was used to detect expression levels of various proteins including CRP78, Mcl-1 . Bc1-2 and E2F-1. The expression of Mcl-1 at mRNA level was monitored by quantitative RT-PCR. cDNA ( pcDNA-E2F-1-Flag )transfection was employed to over-express E2F-1 in cells. Results Gastric adenocarcinoma cells were in general resistant to apoptosis induced by the ER-stress inducer tunicymicin ( TM ) which was associated with up-regulation of Mcl-1 in protein and mRNA levels. Intriguingly , the transcription factor E2F-1 known to repress transcription of Mcl-1 was decreased by exposure to TM. Over-expression of E2F-1 attenuated up-regulation of Mcl-1 in gastric adenocarcinoma cells under ER stress. Conclusion Up-regulation of Mcl-1 may play a role in resistance of gastric adenocarcinoma cells to ER stress-induced apoptosis. This is , at least in part , due to down-regulation of E2F-1 in the cells upon ER stress.关键词
胃肿瘤/腺癌/E2F1转录因子/内质网/衣霉素/细胞凋亡/转染分类
医药卫生引用本文复制引用
王丽,侯丽丽,吴亚欧,桂丽,张旭东,张林杰..E2F-1在胃腺癌细胞内质网应激反应中对Mcl-1的调控作用[J].安徽医科大学学报,2011,46(4):332-336,5.基金项目
国家自然科学基金(编号:30572118) （编号:30572118）
安徽省自然科学基金(编号:070413077) （编号:070413077）
