安徽医科大学学报2011，Vol.46Issue(4)：396-399,4.
原发性肝癌肿瘤标志物研究进展
Effect of different treatment methods on superovulation of rabbits
摘要
Abstract
Objective To explore an stable and higb efficient superovulation method for rabbits, obtain sufficient high-quality rabbit oocytes, and provid reliable materials for the following relevant test. Methods 30 New Zealand white rabbits randomly divided into 3 groups. As for HMG + HCG group, the female rabbit was subcutaneous injected on its neck for 2 times at intervals of 24 h; and for PMSG + HCG test group, the female rabbit was subcutaneous injected on its neck with PMSG 80 IU/pc; and for FSH + HCG test group, the female rabbit was injected on its neck for 6 times at intervals of 12 h,and total dose was 60 IU/pc. After HMG,PMSG and FSH injection,the intravenous injection through the ear edge vein with HCG 100 IU per rabbit at the given time, the egg recovery rate was calculated, and the superovulation effect of three treatment methods was compared. Results The average number of follicles recovered,hyperaemia follicles on the ovary and ovarian cysts of the three groups had significant differences.The average number of follicles recovered from HMG + HCG group was significantly higher than those from FSH +HCG group and PMSG + HCG group ( P ＜0. 05 ). And the average number of follicles recovered form FSH + HCG group was significantly higher than that from PMSG + HCG group ( P ＜ 0. 05 ). And we found that the average number of hyperaemia follicles and ovarian cysts on the ovary of PMSG + HCG group was significantly higher than those from HMG + HCG group and FSH + HCG group ( P ＜0. 05 ). A part of the oocytes had abnormal morphology( such as first polar body broken, egg week gap, cytoplasm abnormal morphology ) which was recovered form PMSG +HCG group. Conclusion The most efficient superovulation methods is HMG + HCG group on rabbits.关键词
肝肿瘤/肿瘤标记/生物学Key words
rabbits/ superovulation/drug effects/ urofollitropin/ chorionic gonadotropin /follicle stimulating hormone分类
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徐楠..原发性肝癌肿瘤标志物研究进展[J].安徽医科大学学报,2011,46(4):396-399,4.基金项目
973前期研究专项基金(编号:2009CD526411) （编号:2009CD526411）
