植物研究2011，Vol.31Issue(1)：105-108,4.
正交设计优化北重楼ISSR-PCR体系
Optimization of ISSR-PCR System for Paris vertcillata
摘要
Abstract
The optimal ISSR-PCR system for Paris vertcillata was established with orthogonal design.A total of 20 μL ISSR-PCR system contains 1 × buffer, dNTP 175 μmol · L- 1,Primer 0.6 μmol · L-1, Mg2 + 1.4 mmol · L-1,Taq DNA polymerase 2.5 U and 80 ng template DNA.The suitable PCR procedure is one cycle denaturing at 95℃ for 5 min ;40 cycles each involved denaturing at 94℃ for 40 s ,annealing at 55℃ for 45 s,extending at 72℃ for 90 s, one cycle extending at 72℃ for 7 min, and then remaining at 4℃.This optimized system would play an important role in further research of systematology on P.vertcillata and its related species, as well as identification on germplasm, and genetic diversity by ISSR molecular marker.关键词
北重楼/ISSR-PCR/分子标记/正交优化分类
农业科技引用本文复制引用
张龙进,白成科..正交设计优化北重楼ISSR-PCR体系[J].植物研究,2011,31(1):105-108,4.基金项目
中央高校基本科研业务费项目(GK261001)、陕西生省科技计划项目(2007K16-03)和西安市科技计划项目(YF07112)资助 （GK261001）
