临床与实验病理学杂志2011，Vol.27Issue(3)：282-285,4.
干细胞因子对高糖环境下豚鼠膀胱Cajal样间质细胞c-kit mRNA及蛋白表达的影响
Effects of SCF on the expressions of c-kit mRNA and protein of interstitial cells of Cajal-like cells in the bladder of guinea pig in the high glucose environment
摘要
Abstract
Purpose To observe the stem cell factor ( SCF ) intervention on interstitial cells of Cajal-like cells ( ICCs-like ) c-kit mRNA and protein expression in the bladder of guinea pig in the high glucose environment. Methods The ICCs-like cells were isolated from the bladder of guinea pig by collagenase digestion and cultured for 24 h, then medium of 5 mmol/L ( normal control group ), 15mmol/L ( high glucose ) glucose concentration was added and continued to culture for 72 h. The high glucose group was divided into high glucose control group and SCF intervention group ( 50 ng/ml,l00 ng/ml ) and cultured for 24 h and 72 h. Last mRNA expression of c-kit was detected by reverse transcription polymerase chain reaction ( RT-PCR ) and protein expression of c-kit was tested and analyzed by laser scanning confocal microscope. Results Compared to control group, c-kit mRNA and protein expression levels of high glucose were significantly decreased. In 24 h group, c-kit mRNA and protein expression in SCF 50 ng/ml group did not increased significantly than high glucose control group( P ＞ 0. 05 ). However, c-kit mRNA and protein expression in SCF 100 ng/ml group were higher than that in high glucose control group( P ＜ 0. 05 ). In 72 h group , c-kit mRNA and protein expression in SCF50 ng/ml and 100ng/ml group were higher than that in high glucose control group( P ＜ 0. 01 ). Conclusion High concentrations of SCF mav increase the expression of c-kit in interstitial cells of Cajal which have changed in shape and function.关键词
Cajal样间质细胞/c-kit/高糖环境/SCF/c-kit信号通路分类
医药卫生引用本文复制引用
刘永国,王勤章,丁国富,刘新军,周建民,李应龙,王江平,钱彪,杨兰..干细胞因子对高糖环境下豚鼠膀胱Cajal样间质细胞c-kit mRNA及蛋白表达的影响[J].临床与实验病理学杂志,2011,27(3):282-285,4.基金项目
国家自然科学基金(30860281) （30860281）
