肿瘤预防与治疗2011，Vol.24Issue(2)：69-73,5.DOI:10.3969/j.issn.1674-0904.2011.02.001
海芋粗提物对人肝癌细胞的细胞毒和凋亡诱导作用
The Cytotoxic and Apoptotic Effect of Crude Extract of Alocasia Macrorrhiza on Human Hepatocellular Carcinoma Cells
方胜涛 1林采余 1张全波 1王力 1林苹 1张洁 1肖恒怡 1王修杰1
作者信息
- 1. 四川大学华西医院,生物治疗国家重点实验室,老年医学研究室,成都,610041
- 折叠
摘要
Abstract
Objective : To investigate the cytotoxic and apoptotic effect of crude extract of alocasia macrorrhiza on human hepatocellular carcinoma cells and to elucidate its mechanism of activity. Methods : Human normal liver cells ( LO2 ) and hepatocellular carcinoma cells ( SMMC-7721 ) were tested in vitro for cytotoxicity, colony formation inhibition, EdU incorporation, AO/EB staining apoptotic cells, and cell cycle distribution through flow cytometry after treated with crude extract of alocasia macrorrhiza. The expression of PPARγ, Cyclin Dl , Rb , P21, Bax, Bcl-2 mRNA were detected through RT-PCR. Results : After treated hy crude extract of alocasia macrorrhiza, the proliferation ( IC50 =414 μg/ml ) and colony formation of cancer cells decreased ( P ＜ 0. 05 ) in a dose-timedependent manner; EdU labeling index of cancer cells decreased ( P ＜ 0. 05 ); and AO/EB staining apoptotic cells increased ( P ＜ 0. 01 ). The apoptotic index increased ( P ＜0. 01 ) with the cells in G0/G1 phase increased ( P ＜ 0. 05 ) and decreased in S and G2/M phase ( P ＜ 0. 05 ) through FCM analysis ; RT-RCR results showed that the crude extract of alocasia macrorrhiza treatment significantly increased the mRNA expression levels of PPARγ ( P ＜0. 01 ), Rb ( P ＜ 0. 01 ) and Bax( P ＜ 0. 01 ) genes, and decreased expression levels of Cyclin D1 ( P ＜0. 01 ), and Bcl-2 ( P ＜ 0. 05 ) genes. Conclusion: The findings in this experimental study suggest that thecrude extract of alocasia macrorrhiza has potential cytotoxic and apoptotic effect on human hepatocellular carcinoma cells, its mechanism of activity might be associated with the inhihition of DNA synthesis, cell cycle ( G0/G1 ) arrest, apoptosis induction and up-regulation the expressions of PPARγ, Rb, and Bax genes and downregulation of the expressions of Cyclin Dl and Bcl-2 genes.关键词
海芋/粗提物/肝癌细胞/细胞毒/凋亡分类
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方胜涛,林采余,张全波,王力,林苹,张洁,肖恒怡,王修杰..海芋粗提物对人肝癌细胞的细胞毒和凋亡诱导作用 [J].肿瘤预防与治疗,2011,24(2):69-73,5.
