中国组织工程研究与临床康复2011，Vol.15Issue(8)：1377-1380,4.DOI:10.3969/j.issn.1673-8225.2011.08.011
胶原-壳聚糖支架材料与间充质干细胞的组织相容性
Histocompatibility of bone marrow mesenchymal stem cell and collagen-chitosan scaffold
摘要
Abstract
BACKGROUND: Recent studies have showed that the collagen-chitosan complex scaffold can be used as nerve tissue engineering scaffold, but little evidence is available in the histocompatibility study.OBJECTIVE: To observe rabbit bone marrow mesenchymal stem cells (MSCs) growth and differentiation in the surface of collagen-chitosan complex scaffold.METHODS: Rabbit MSCs were isolated and cultured in serum-free medium. Flow cytometry was used to analyze the immunophenotype. The MSCs were planted on the gel scaffold (experimental group) and polylysine coated coverslip (control group), the cells were cultured in neural induction medium. The cell differentiation and growth characteristics were observed by inverted phase contrast microscope.RESULTS AND CONCLUSION: The phenotype was CD29+, CD44+, CD166+. Inverted phase contrast microscope showed that:the MSCs grew well with the prominence process seen at 7 days in experimental group. The cells growth characteristics had no difference with the control group. The MSCs and collagen-chitosan complex scaffold have good histocompatibility.关键词
胶原蛋白/壳聚糖/骨髓间充质干细胞/组织工程/组织相容性/支架材料分类
医药卫生引用本文复制引用
李晓龙,穆长征,马云胜..胶原-壳聚糖支架材料与间充质干细胞的组织相容性[J].中国组织工程研究与临床康复,2011,15(8):1377-1380,4.基金项目
辽宁省教育厅创新团队项目基金(2006T062) （2006T062）
辽宁省自然科学基金(20072201). （20072201）
