中国组织工程研究与临床康复2011，Vol.15Issue(8)：1409-1412,4.DOI:10.3969/j.issn.1673-8225.2011.08.019
体外自组装IKVAV纳米纤维凝胶在二维体系中对神经祖细胞分化的影响
Effect of self-assembling nanofiber gel containing IKVAV in two-dimensional culture system on differentiation of neural progenitor cells in vitro
摘要
Abstract
BACKGROUND: Numerous studies have demonstrated that the self-assembling nanofiber promotes neural progenitor cells proliferation and differentiation.OBJECTIVE: To study the differentiation of neural progenitor cells on self-assembling nanofiber gel containing IKVAV in two-dimensional culture system in vitro.METHODS: Neural progenitor cells of SD neonatal rats were cultu red in culture medium and examined by immunofluorescence.Amphipathic polypeptide solution containing 1 ％ IKVAV-PA was trigger to form three-dimensional porous gel by the addition of DMEM/F12, and the structure was detected under transmission electron microscope. Neural progenitor cells were seeded on the surface of coverslips covered with 1 ％ KVAV-PA or covered with 0.1 g/L polylysine, and the cell differentiation was determined by neurofilament and glial fibrillary acidic protein after cultivated 1, 3, 7 days respectively.RESULTS AND CONCLUSION: The cultured Nestin-positive cells were found, and they were able to differentiated into neurofilament-positive neurons and glial fibrillary acidic protein-positive glial cells, confirming as neural progenitor cells;Transmission electron microscopy showed that the gel derived from amphipathic polypeptide solution containing IKVAV-PA, was composed of nanofiber network, their diameter ranging 7.0-8.0 nm and the length 100-1500 nm. The ability of differentiation into neurons of the amphipathic polypeptide solution containing IKVAV-PA surpasses to the polylysine. The self-assembling nanofiber gel contained IKVAV in two-dimensional culture system may promote the differentiation of neural progenitor cells in vitro.关键词
神经祖细胞/纳米支架/IKVAV/纳米纤维凝胶/生物材料与纳米技术分类
医药卫生引用本文复制引用
扈佃磊,于圣会,刘宁,盛伟斌,郭海龙,马艳,毕晓娟..体外自组装IKVAV纳米纤维凝胶在二维体系中对神经祖细胞分化的影响[J].中国组织工程研究与临床康复,2011,15(8):1409-1412,4.基金项目
新疆医科大学第一附属医院青年专项基金项目(20009QN06). （20009QN06）
