中国组织工程研究与临床康复2011，Vol.15Issue(8)：1449-1453,5.DOI:10.3969/j.issn.1673-8225.2011.08.029
微囊化细胞的低温保存
Cryopreservation of microencapsulated cells
摘要
Abstract
BACKGROUND: Microcapsule is an effective tool for immunoisolation, cryopreservation makes the preservation and the transportation of the microcapsules easy, which is helpful for microencapsulated technology applied in clinical practice.OBJECTIVE: To summarize the cryopreservation of microencapsulated cells, such as cryopreservation characteristic of microencapsulated cells, advantages and disadvantages of different cryopreservation methods, research methods and so on.METHODS: A computer-based online search of PubMed database (from 1980 to 2010), Wanfang database (from 1991 to 2010)and Vip database (from 1991 to 2010) was performed for articles related to the cryopreservation of microencapsulated cells,cryopreservation technology and devices, cryopreservation application in medical field .RESULTS AND CONCLUSION : The cryodamage characteristic of microencapsulated cells are different to the cells and tissues.The relatively large size of microcapsules and complex structure of semi-permeable membrane make it susceptibly that microcapsule structure and cells in microcapsules are damaged by ice crystals and solution in cryopreservation. So the cryopreservation protocol of cell suspension could not be used in microencapsulated cells cryopreservation. Slow-cooling and vitrification are two common methods of the cryopreservation of microencapsulated cells, each has advantages and disadvantages. The technology of cryopreservation of microencapsulated cells is not yet mature. The mechanism underlying the cryopreservation of microencapsulated cells needs further strengthen at the same time of exploring the optimal cryopreservation protocols for microencapsulated cells and new cryopreservation facilities for microencapsulated cells.关键词
低温保存/微囊化细胞/玻璃化/移植/胰岛移植/生物材料分类
医药卫生引用本文复制引用
叶萍,常兆华,刘宝林,赵庆孝,彭承宏,沈柏用,韩宝三..微囊化细胞的低温保存[J].中国组织工程研究与临床康复,2011,15(8):1449-1453,5.基金项目
国家"十一五"高新技术研究发展计划-"863"项目(2008AA02Z417):微粒生物人工肝反应器构建的研究 （2008AA02Z417）
国家自然科学基金项目(30801107):微粒 生物人工胰构建的研究 （30801107）
国家自然科学基金项目(30772105):新型超分子自组装微囊/肝细胞球形聚集体构建的研究 （30772105）
国家自然科学基金项目(30672043):微粒肝细胞生物反应器-微囊化三维多孔微载体构建的研究 （30672043）
2005/2008 国家自然科学基金重点项目(20434030):智能化微囊的制备、特异识别与填充释放 （20434030）
国家自然科学基金项目(20074031):人肝细胞/聚合物杂化界面和生物人工肝的研究 （20074031）
上海市教委科研创新项目(09YZ84):新型 超分子自组装半透膜微囊胰岛移植的实验研究 （09YZ84）
上海市科委自然科学基金项目(07ZR14076):超分子自组装可控半透膜/肝细胞球形聚集体构建的研究 （07ZR14076）
上海市科委自然科学基金项目(09140902300):微囊化人胰腺癌细胞建立同位移植瘤裸鼠动物模型的研究 （09140902300）
山东省科技攻关计划(2008GG10002027):微粒生物人工肝反应器构建的研究 （2008GG10002027）
上海市研究生创新基金项目(JWCXSL1021):乳腺癌冷冻消融精准治疗技术研究. （JWCXSL1021）
