水生生物学报2011，Vol.35Issue(1)：132-137,6.DOI:10.3724/SP.J.1035.2011.00132
尼罗罗非鱼消化道肥大细胞的组化性质
STUDY ON THE HISTOCHEMICAL PROPERTY OF THE MAST CELLS IN THE DIGESTIVE TRACT OF NILE TILAPIA
摘要
Abstract
Five healthy nile tilapia (average weight 500g) were bought. After executing, the esophagus, stomachus (stomachus fundus, stomachus pylorus and stomachus cardiacus), small intestine (foregut, midgut and hindgut) were respectively fixed in the Carnoy solution (60％ dehydrate alcohol, 30％ chloroform and 10％ glacial acetic acid), continuously embedded and sliced with 6μm. Toluidine blue staining (MTB), Alcian blue Safranin O staining (AB/SO),methyl green-Pyronine staining (MG-P), Azure II - Eosin - Wright mixture staining and Thionine staining were used to investigate the histochemical property of the mast cells in the digestive tract of tilapia.The mast cells were showed in the esophagus, stomachus and small intestinal wall of nile tilapia. The mast cells in the slides of esophagus and stomachus mostly distributed between lamina propria and stomachus gland. The mast cells in intestinal tract mainly distributed in lamina propria and under intestine epithelium, while less in connective tissue of submucosa. They were circular, ellipse and spindle, and intended to be around blood vessels. The results of five histochemical stainings were as follows: The MTB, AB/SO and MG-P stainings could show the mast cells better, especially the AB/SO was the best among them. The mast cells were distinctly outlined, the cytoplasmic particle of which was clear. The similarity of the research on teleostean mast cells from home and abroad was that the mast cells of nile tilapia was similar to mucosal mast cell of mammal. The effect of MG-P staining was good too, in which the cytoplasmic particle of mast cells showed red, which meant the cytoplasmic contained mainly heparin not histamine. It confirmed further that the histochemical property of the mast cells of nile tilapia was similar to mucosal mast cell of mammal. The effect of Azure II - Eosin - Wright mixture staining was better, but the mast cells dyed was few. Whereas, the mast cells in the sections of digestive tract of nile tilapia could not been showed by 80％ Alcohol-Thionine staining. The reason might be different stationary liquid or the difference in histochemical property of mast cells of nile tilapia, carp and white fish. The observation showed that the mast cells in the digestive tract of nile tilapia mostly distributed in the points which easily revealed from environment antigen such as shallow mucosa or blood vessels, the connective tissue around gland and so on. The mast cells had similar distribution characteristics in different organs of other animals. From the above, the distribution characteristics of creatural mast cells were closely bound up with the function of cell, which maybe provide handy positional foundation for the exertion of autoimmune function in a sense. The experiment also found that the location of mast cells in connective tissue of mucosa in digestive tract of nile tilapia were similar with most of the vertebrate, of which the location was intended to be around blood vessels. It meant that teleostean mast cells were closely related to blood vessels as mammals were.It was reported that the growth of vascular endothelial cells and constitute of blood circulation were affected by histamine and heparin released from mast cells. The vascular endothelial leukocyte adhesion molecule 1, which could watch blood stream by attracting lymphocyte adhere to the inner wall of blood vessels, could be induced by Tumor Necrosis Factor-α (TNF-α) released from mast cells in human skin. It was obviously that the mast cells around blood vessels played an important role in reaction of blood vessels.关键词
尼罗罗非鱼/肥大细胞/组织化学分类
生物科学引用本文复制引用
林旋,林树根,王全溪,陈梅芳,严美姣,王寿昆..尼罗罗非鱼消化道肥大细胞的组化性质[J].水生生物学报,2011,35(1):132-137,6.基金项目
福建省自然科学基金项目(2008J0249) （2008J0249）
福建农林大学校青年教师基金(07B13) （07B13）
农业公益性行业科研专项经费项目子项目(20080313)资助 （20080313）
