水生生物学报2011，Vol.35Issue(2)：256-261,6.DOI:10.3724/SP.J.1035.2011.00256
水体pH胁迫对异育银鲫皮质醇激素和非特异性免疫的影响
EFFECTS OF pH STRESS ON CORTISOL AND NON-SPECIFIC IMMUNITY OF CARASSIUS AURATUS GIBELIO
摘要
Abstract
Effects of pH stress on cortisol and non-specific immunity of Carassius auratus gibelio were investigated.Acidity and Alkalinity of the aquaculture water were maintained at pH 6.0, 7.4, 8.5 and 9.5, where pH 7.4 was used as control. pH value of the aquaculture water was adjusted by HCl (1 N) or NaOH (1 N). Fish blood was sampled at 0, 14 and 30 days during the experiment. Leucocytes isolated by 55％ percoli were used for determination of phagocytosis and respiratory burst activity by flow cytometry, and the plasma was used to survey the cortisol concentration and lysozyme activity. Fish weight was measured when pH stress protocol finished. The fish was then challenged by the bacteria Flavobacterium columnare, and dead fish was recorded after 7 days of the challenge. Under the stress of pH 6.0, phagocytosis of leucocytes decreased significantly at the 14 and 30 day, but no significant differences were found in respiratory burst activity and cortisol level. There were significant differences in lysozyme activity and growth between pH 6.0 and control group. No fish died from the bacteria challenge. Under pH 8.5 and 9.5 stress, the cortisol concentration increased significantly, and phagocytosis and respiratory burst activity decreased significantly at the 14 and 30 day. The challenge of bacteria resulted in fish death. The results suggested that non-specific immunity and growth of the carp was heavily affected by the pH 8.5 and 9.5 stress, and phagocytosis and respiratory burst activity of fish leucocytes determined by flow cytometry could assess the influence of stress on fish health.关键词
pH胁迫/异育银鲫/皮质醇/溶菌酶活性/吞噬活性/呼吸暴发/流式细胞术分类
生物科学引用本文复制引用
李文祥,谢骏,宋锐,邹红,吴山功,王桂堂..水体pH胁迫对异育银鲫皮质醇激素和非特异性免疫的影响[J].水生生物学报,2011,35(2):256-261,6.基金项目
现代农业产业技术体系建设专项资金资助(NYCYTX-49-15) （NYCYTX-49-15）
国家重点基础研究发展计划(973)项目(2009CB118705)资助 （973）
