水生生物学报2011，Vol.35Issue(2)：283-290,8.DOI:10.3724/SP.J.1035.2011.00283
棉子糖对花鲈生长、免疫抗应激及抵御嗜水气单胞菌攻毒的影响
EFFECTS OF RAFFINOSE ON GROWTH PERFORMANCE, IMMUNITY, STRESS RESPONSE AND SURVIVAL OF JAPANESE SEABASS(LATEOLABRAX JAPONICUS)CHALLENGED WITH AEROMONAS HYDROPHILA
摘要
Abstract
A 16w growth trial was conducted to study the effects of raffinose on growth performance, non-specific immunity, stress response and survival of Japanese seabass (Lateolabraxjaponicus) challenged with pathogenic bacteria,Aerornonas hydrophila in a recirculation system.Five experimental diets with 0, 200, 400, 800 and 2000 mg/kg of raffinose supplements were prepared, and named as R0, R200, R400, R800 and R2000, respectively.Each diet was fed to triplicates groups of Japanese seabass (initial body weight: average of 3g), with 30 fish in each tank.At the end of trial,fish were weighed and serum sample were collected.Besides, a 30s air-exposure stress test and a challenge test with A.hydrophila were carried out.Survival and feed intake of fish did not show significant difference among all groups (P＞0.05).Weight gain rate (WGR) of R800 group was significantly higher than that of control, R400 and R2000 groups (P ＜ 0.05).Feed Conversion Ratio (FCR) and protein efficiency rate (PER) of R800 treatment were significantly lower and higher than R400 treatment, respectively (P ＜ 0.05).Condition factor (CF) of control, R200 and R2000 treatments were significantly higher than R400 and R800 treatments (P ＜ 0.05).There were no difference on serum lysozyme, superoxide dismutase and malondialdehyde (P ＞ 0.05), and glucose and cortisol level of serum after 30s air stress were no difference (P ＞ 0.05), either.However, accumulative survival of control group significantly lower than that of groups with various level of raffinose after being challenged by Aerornonas hydrophila (P ＜ 0.05).800mg/kg of raffinose was recommended to use in diet of Japanese sea bass.关键词
花鲈/棉子糖/非特异性免疫/抗应激/嗜水气单胞菌分类
农业科技引用本文复制引用
葛红云,王兰梅,郑银桦,薛敏,赵维香,吴秀峰,胡亮,王嘉..棉子糖对花鲈生长、免疫抗应激及抵御嗜水气单胞菌攻毒的影响[J].水生生物学报,2011,35(2):283-290,8.基金项目
"十一五"国家支撑计划项目(2006BAD12B06,2006BAD12B08)资助 （2006BAD12B06,2006BAD12B08）
