中国组织工程研究与临床康复2011，Vol.15Issue(8)：1336-1340,5.DOI:10.3969/j.issn.1673-8225.2011.08.002
生物可降解聚合物结合碱性成纤维细胞生长因子与内皮细胞的黏附
Adhesion of endothelial cells with biodegradable polymer and basic fibroblast growth factor
摘要
Abstract
BACKGROUND:The application of biodegradable materials is the important research component of small-caliber tissue engineered vessels in vitro. How to modify degradable materials so as to facilitate in vitro anticoagulant and promote endothelial cell adhesion, is one of the hotspots in the study of vascular tissue engineering.OBJECTIVE:To in vitro load basic fibroblast growth factor (bFGF) by using biodegradable polycaprolactone (PCL) graft heparin materials, and to observe the effects on endothelial cell adhesion.METHODS:Applying PCL biodegradable materials, the activated heparin esterified with PCL hydroxyl end and then anchored at both ends of the PCL. The vascular scaffold was prepared with electrospinning technique. Using the electrostatic adsorption between heparin and growth factors, the scaffold was loaded with bFGF. After low-density short-term static cultivation of endothelial cells, the bFGF-loaded biodegradable PCL on the adhesion of endothelial cells was investigated.RESULTS AND CONCLUSION:The bFGF-loaded biodegradable PCL graft heparin scaffold is successfully fabricated.Endothelial adhesion experiments demonstrated that the biodegradable scaffold was conducive to endothelial cell adhesion.Biodegradable PCL graft heparin material loading bFGF is beneficial to endothelial cell adhesion in vitro.关键词
可降解/聚已内酯/肝素/支架/内皮细胞/碱性成纤维细胞生长因子/组织工程分类
医药卫生引用本文复制引用
武欣,冯增国,汪忠镐,谷涌泉,张建,叶霖,段红永,吴英峰,陈兵,李建新,张淑文..生物可降解聚合物结合碱性成纤维细胞生长因子与内皮细胞的黏附[J].中国组织工程研究与临床康复,2011,15(8):1336-1340,5.基金项目
国家高技术研究发展计划(863计划)资助项目:" 小口径组织工程血管的基础及应用研究"(2006AA02A134) （863计划）
北京市自然科学基金资助项目:多孔脱细胞小口径组织工程动脉支架及其再细胞化研究"(5082007)和"在体靶向自组装小口径组织工程动脉的初步研究"(2103049). （5082007）
