江苏大学学报（医学版）2011，Vol.21Issue(1)：18-22,5.
德国小蠊变应原BgGSTD1的抗原表位特征及三维结构建模
Characterization of antigenic epitopes of BgGSTD1 from Blattella germanica and its three dimensional structure modeling
摘要
Abstract
Objective : We conducted bioinforrnatic analyzes to identify and characterize the structural properties of Delta-glutathione S -transferase ( BgGSTD1) from Blattella germanica. , which gave the clue for the diagnosis and therapy of cockroach allergy . Methods: BLAST was applied to search for the proteins ,which show high similarities with BgGSTD 1. Clustalx 1. 83 was used for sequence alignment and phylogenetic tree construction. Antigenic epitopes of BgGSTD1 were predicted by the software of DNA Star. The motif and three -dimensional structure modeling were analyzed by online software in Scan Prosite and SWISS -MODEL web server. Results: The phylogenetic analysis found that BgGSTD 1 showed highest similarity with the glutathione S-transferase identified from Periplaneta americana. BgGSTD1 was a global protein , mainly containing α＋β secondary structure. Its antigenic epitope were mostly located on 9 - 10, 58 -61 , 79 -81, 86 - 87 , 179 - 180 , 189 - 190 , 216 regions. Motif analysis found that BgGSTD 1 had a soluble glutathione S-transferase N-terminal domain and a soluble glutathione S -transferase C-terminal domain. Threedimensional structure of BgGSTD1 was also constructed. Conclusion: It seemed that the predicted structure could reflect the real tertiary structure of BgGSTD 1 , which was useful for the further study of structure -func-tion relationship .关键词
德国小蠊/谷胱甘肽转移酶/抗原表位/三维结构建模分类
生物科学引用本文复制引用
张伟,胡玉静,金珊珊,杨海伟,魏继福,何韶衡..德国小蠊变应原BgGSTD1的抗原表位特征及三维结构建模[J].江苏大学学报（医学版）,2011,21(1):18-22,5.基金项目
国家自然科学基金资助项目(30801016 （）
30972822 （）
81001329 （）
81030054) （）
