江苏大学学报（医学版）2011，Vol.21Issue(1)：29-33,41,6.
SsrB对伤寒沙门菌氧应激早期基因表达的调节
Regulation of ssrB on gene expression of Salmonella enterica serovar Typhi at early stage of oxidative stress
摘要
Abstract
Objective : To investigate the regulation of ssrB on gene expression of Salmonella enterica serovar Typhi (S. Typhi) at early-stage of oxidative stress. Methods: The ssrB deleted mutant of S. Typhi was generated through homologous recombination mecliated by suicide plasmid ; the gene expression profiles of the wild-type strain and the ssrB deleted mutant at early -stage of oxidative stress was investigated by genormc microarray assay; qRT-PCR was performed to further validate the results of microarray assay . The HeLa cells were invacled by S. Typhi to explore the influence of ssrB on invasion of epithelial cells. Results: The ssrB deleted mutant of S. Typhi was preparecl successfully ; analysis of genomic assay showed that , compared to the wild -type strain , 68 genes were up -regulated and 20 genes were down -regulated in the ssrB deleted mutant at early stage of oxidative stress . The results of qRT-PCR assay were consistent with the results of microarray assay. The ability of the ssrB deleted mutant to invade the epithelial cells was only 34. 6％ of the wilcl-type strain. Conclusion: SsrB of S. Typhi played an important role in regulating gene expression at early stage of oxidative stress and enhanced the ability of S. Typhi to invade epithelial cells.关键词
伤寒沙门菌/ssrB/氧应激/基因芯片/侵袭分类
医药卫生引用本文复制引用
王敏,罗哲,杜鸿,孟彦辰,王菲,倪斌,徐顺高,黄新祥..SsrB对伤寒沙门菌氧应激早期基因表达的调节[J].江苏大学学报（医学版）,2011,21(1):29-33,41,6.基金项目
国家自然科学基金资助项目(30870095) （30870095）
