中国组织工程研究与临床康复2011，Vol.15Issue(6)：995-999,5.DOI:10.3969/j.issn.1673-8225.2011.06.011
脐血浆神经营养活性蛋白成分对CD34+细胞增殖分化的影响
Effects of neurotrophic factors in umbilical cord blood plasma on CD34+ cell proliferation and differentiation
张丽 1李倩如 2杜英 2杨波 2李国喜 3胡祥4
作者信息
- 1. 河南省红十字血液中心,河南省郑州市,450053
- 2. 郑州大学基础医学院微生物学与免疫学教研室,河南省郑州市450001
- 3. 郑州大学第一临床医院神经外科,河南省郑州市,450052
- 4. 深圳市北科细胞工程研究所,广东省深圳市,518000
- 折叠
摘要
Abstract
BACKGROUND:Further studies are needed to understand if the neurotrophic factors in human umbilical cord blood plasma have the promoting effect on neural regeneration and repair.OBJECTIVE: To investigate the component of neurotrophic factors in umbilical cord blood plasma, observe its application in neural regeneration and repair.METHODS: The difference was compared between the human umbilical cord blood plasma (HUCBP) and peripheral venous blood plasma (PVBP) from healthy voluntary womenfolk using human antibody array. The CD34+ cells were sorted from human umbilical cord blood using immunomagnetic beads. Following cultivating in vitro and counting, proliferative ability of CD34+ cells was observed. Cell differentiation was observed using immunohistochemical method.RESULTS AND CONCLUSION: The concentrations of 31 kinds of protein molecules were significantly higher in HUCBP than PVBP. Of them, 10 kinds of molecules thereinto have significant potential of nerve regeneration and plasticity, including FGF4,Frizzled-3, IL-3, RAGE, CRIM-1, Neuritin, Neuropilin-2, Neurturin, SFRP-3 and Tomoregulin-1. HUCBP may promote the CD34+ cells proliferation in cell culture medium and differentiation into nerve cells.关键词
脐血浆/CD34+细胞/神经营养因子/FGF4/Frizzled-3/Neutrin分类
医药卫生引用本文复制引用
张丽,李倩如,杜英,杨波,李国喜,胡祥..脐血浆神经营养活性蛋白成分对CD34+细胞增殖分化的影响[J].中国组织工程研究与临床康复,2011,15(6):995-999,5.
