中国组织工程研究与临床康复2011，Vol.15Issue(6)：1015-1019,5.DOI:10.3969/j.issn.1673-8225.2011.06.015
干细胞相关因子在大肠癌组织及SW620细胞株的表达
Expression of stem cell-associated genes in colorectal cancer and SW620 cell line
摘要
Abstract
BACKG ROUND: Tumor cells always show some stem cell markers. The expression of these markers will help us to understand the mechanism of tumorgenesis and to perfect the theory of tumor stem cells.OBJECTIVE: To investigate the expression of stem cell-related molecules markers CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 in colorectal cancer tissue, the difference compared with the expression of adjacent tissues, as well as the difference in expression of above-described genes in sorted CD133+ SW620 cell line.METHODS: RNA was extracted from cancer tissues of 29 colon cancer tissue specimens. The expression of CD133, CD166,Oct4, Sox2, C-myc, Klf4 and Bmi-1 was detected by RT-PCR in colon cancer tissues and adjacent tissues. CD133+ SW620 cells were sorted, and RNA was extracted from CD133+ cells and CD133- cells. The different expressions of above genes were detected using RT-PCR.RESULTS AND CONCLUSION : All samples were proved to be poorly/moderately differentiated adenocarcinoma or myxoadenocarcinoma in 29 cases. The relative quantity expressions of the stem cell-related molecules markers in the cancer tissue were higher than adjacent tissues (P ＜ 0.05). RT-PCR indicated that stem cell associated genes expressions (CD133,CD166, Oct4, Sox2, C-myc, Klf4, Bmi-1) in CD133+ sorted cells were higher than that in CD133- ones. These suggested that CD133, CD166, Oct4, Sox2, C-myc, Klf4 and Bmi-1 were used in colorectal cancer diagnosis.关键词
大肠癌/肿瘤干细胞/CD133/CD166/Oct4/Sox2/Klf4/C-myc/Bmi-1分类
医药卫生引用本文复制引用
李秋娴,黄颖峰,魏亚明,王晓华,罗宏图,杨邵宇,许艳丽,黄于艺,叶亨..干细胞相关因子在大肠癌组织及SW620细胞株的表达[J].中国组织工程研究与临床康复,2011,15(6):1015-1019,5.基金项目
广东省医学科研基金(A2008517).课题名称:消化道肿瘤患者外周血中癌细胞、癌干细胞检测分离与临床应用 （A2008517）
广州市科技计划项目(2008J1-C171).课题名称:大肠癌实体与外周血癌干细胞分离及其Bmi-1基因表达调控. （2008J1-C171）
