中国组织工程研究与临床康复2011，Vol.15Issue(6)：1020-1023,4.DOI:10.3969/j.issn.1673-8225.2011.06.016
自体外周血干细胞动员和采集并联合化疗方案治疗儿童神经母细胞瘤及原始神经外胚层肿瘤35例
Mobilization and collection of autologous peripheral blood stem cells combined with chemotherapy regimen for treatment of neuroblastom/primitive neuroectodermal tumor in 35 children
张伟令 1张谊 1黄东生 1杨怡平 1刘晓超 2吴怡平2
作者信息
- 1. 首都医科大学附属北京同仁医院儿科,北京市,100176
- 2. 首都医科大学附属北京同仁医院中心实验室,北京市,100176
- 折叠
摘要
Abstract
BACKGROUND: Autologous peripheral blood stem-cell transplantation (APBSCT) is an important method to treat malignant solid tumor in children. Mobilization and collection of stem cells are the key for hematopoietic reconstitution.OBJECTIVE: To evaluate the clinical effect of mobilization and collection by cyclophosphamide, doxorubicin and vincristine mobilization chemotherapy program in children with neuroblastom/primitive neuroectodermal tumor (NB/PNET).METHODS: Protocol of cyclophosphamide, doxoru bicin and vincristine mobilization was used as mobilization chemotherapy in 35 cases (including 30 NB and 5 PNET). Effect of stem cell collection was observed.RESULTS AND CONCLUSION: Leukocytes decreased to less than 2×109/L on 49 days (averagely 6.5 days) after the chemotherapy when granulocyte-colony stimulating factor (G-CSF) 5-10 mg/kg was given to stimulate hematopoiesis. Collection began when leukocytes reached to more than 5×109/L on 13-19 days after chemotherapy (averagely 15.5 days). Mononuclear cells and CD34+ cells were successfully collected in all cases. Total collection times were 1-4 times, averagely 2.1 times. The volumes of mononuclear cells and CD34+ cells were respectively (6.1±1.2)X108/kg and (5.3+0.8)×106/kg. Trypan blue exclusion rate was 99.5％(99％-100％). No severe complication was observed during the mobilization. All subjects could tolerate. Prompt and sustained hemocytoblastic reconstitution was observed in 25 children. Leukocytes began to increase (neutrophilic granulocytes ＞ 0.5×109/L) at 10-20 days posttransplantation (averagely 14 days). Hemoglobin recovered (＞ 80 g/L) at 10-30 days posttransplantation (averagely 18 days). Platelet recovered (＞ 20×109/L) at 12-35 days posttransplantation (averagely 20 days).The program of cyclophosphamide, doxorubicin and vincristine is a highly efficient autologous peripheral blood stem cell mobilization and collection protocol in children with NB/PNET.关键词
神经母细胞瘤/动员/儿童/自体外周血干细胞/原始神经外胚层肿瘤分类
医药卫生引用本文复制引用
张伟令,张谊,黄东生,杨怡平,刘晓超,吴怡平..自体外周血干细胞动员和采集并联合化疗方案治疗儿童神经母细胞瘤及原始神经外胚层肿瘤35例[J].中国组织工程研究与临床康复,2011,15(6):1020-1023,4.
