中国组织工程研究与临床康复2011，Vol.15Issue(6)：1030-1034,5.DOI:10.3969/j.issn.1673-8225.2011.06.018
骨髓间充质干细胞对慢性哮喘小鼠血清白细胞介素12和白细胞介素4水平的影响
Effects of bone marrow mesenchymal stem cells on the serum levels of interleukin-4 and interleukin-12 in chronic asthmatic mice
摘要
Abstract
BACKGROUND: Previous studies have demonstrated that anti-inflammatory actions of bone marrow mesenchy (BMSCs)couldameliorate severity of acute lung injury.However,only a few studies have addressed BMSCs transplantation for asthma.OBJECTIVE: To study the effect of allogeneic BMSCs transplantation on the serum levels of interleukin-12 (IL-12) and IL-4 in chronic asthmatic mice.METHODS: Forty female BALB/c mice were equally randomized into four groups. Mice of normal control and BMSCs control groups received PBS sensitization to arouse mice. On day 21 before suscitation, PBS or BMSCs (30 μL) were injected via the intratracheal injection. Mice of asthma model and BMSCs treatment groups were used to establish chronic asthma using chicken ovalbumin. On day 21 before suscitation, PBS or BMSCs (30 μL) were injected via the intratracheal injection. Serum inflammatory factor levels were measured in mice of each group using enzyme linked immunosorbent assay.RESULTS AND CONCLUSION : There were a great number of inflammatory cell infiltrations around airway and vessel, in conjunction with epithelial proliferation and partial breakage and defluvium, patchy airway occlusion by hyperviscous mucus and hypertrophy and hyperplasia of airway smooth muscle in the asthma model group. However, there was no obvious inflammatory cells infiltration in the airway and airway remodeling both in normal control group and BMSCs control group. Airway inflammation and airway remodeling were significantly relieved in the BMSCs treatment group. Compared to the normal control group and BMSCs control group, low level of IL-12 and high levels of IL-13 and IL-4 were present in the asthma model group. Transplantation of BMSCs significantly elevated level of IL-12 and decreased level of IL-4 in serum. Results suggest that BMSCs for asthma can relieve airway inflammation and airway remodeling by decreasing IL-4 levels and increasing IL-12 levels.关键词
哮喘/骨髓间充质干细胞/白细胞介素12/白细胞介素4/气道局部移植分类
医药卫生引用本文复制引用
戈霞晖,白冲,陈若华,胡珍丽,朱天怡,李强..骨髓间充质干细胞对慢性哮喘小鼠血清白细胞介素12和白细胞介素4水平的影响[J].中国组织工程研究与临床康复,2011,15(6):1030-1034,5.基金项目
国家自然科学基金(30871133).课题名称:骨髓间充质干细胞移植对慢性阻塞性肺病气道及肺损伤的修复作用及相关机制. （30871133）
