中国组织工程研究与临床康复2011，Vol.15Issue(6)：1035-1039,5.DOI:10.3969/j.issn.1673-8225.2011.06.019
骨髓间充质干细胞移植急性一氧化碳中毒迟发性脑病大鼠星形胶质细胞及髓鞘的变化
Changes in astrocytes and myelin sheath in rat models of delayed encephalopathy due to acute carbon monoxide poisoning following bone marrow mesenchymal stem cells transplantation
摘要
Abstract
BACKGROUND: The therapy of delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) in hyperbaric oxygen.They all cost higher and have long low effect.OBJECTIVE: To first observe the changes of astrocytes and nerve myelin before and after bone marrow mesenchymal stem cells (BMSCs) transplantation to treat the DEACMP.METHODS: Sprague-Dawley rats were divided randomly into three groups: control group, sham operated group, and stem cell transplantation group. All rats were injected carbon monoxide into abdominal cavity to produce DEACMP models.The transplantation group was injected with allograft bone marrow stromal cells through the left carotid artery at 0, 3,6,12,24,72 hours, 1 week after model establishment. External carotid artery was occluded in the sham operated group. The sham operated group was injected the same amount of phosphate buffered saline, and the control group was not injected anything.We detected the changes of glial fibrillary acidic protein (GFAP) by immunohistochemistry and observed myelin through Fast Green-FCF staining at 1, 2, 3 and 4 weeks posttransplantation.RESULTS AND CONCLUSION: The expression of GFAP in the rat brain in stem cell transplantation group was significantly higher than that of control group and the sham operated group at 6, 12 and 24 hours (P ＜ 0.05). No significant difference in GFAP expression of each group was detected at 1-4 weeks (P ＞ 0.05). The average absorbance of myelin in stem cell transplantation group was significantly higher at 6, 12 and 24 hours than control and sham operated groups (P ＜ 0.05); the average absorbance of myelin of stem cell transplantation group at 2 weeks were significantly higher than that at 1 , 3, 4 weeks (P ＜ 0.05).These indicated that transplanted BMSCs into the DEACMP rats models through the left carotid artery could increase the responsivity of GFAP and enhance remyelination. The best time for transplantation was between the 6 hours and the 24 hours and the positive effects were significantly observed between 1 week and 2 week after transplantation.关键词
一氧化碳中毒迟发性脑病/骨髓间充质干细胞/胶质纤维酸性蛋白/髓鞘/干细胞移植分类
医药卫生引用本文复制引用
邢红霞,殷闯,王玉梅,刘胜,史莉瑾,李杰..骨髓间充质干细胞移植急性一氧化碳中毒迟发性脑病大鼠星形胶质细胞及髓鞘的变化[J].中国组织工程研究与临床康复,2011,15(6):1035-1039,5.基金项目
2007年河南省科技厅立项(072102310077)项目. （072102310077）
