江苏大学学报（医学版）2011，Vol.21Issue(1)：26-28,49,封3,5.
miR-133a真核表达载体的构建及转染大鼠心肌H9C2细胞
Construction of miR-133a eukaryotic expression vector and transfection into H9C2 cell
张洪涛 1张赢予 2周艳芳 2王好 2郑枫 2张国辉2
作者信息
- 1. 江苏大学临床医学院,江苏,镇江,212001
- 2. 江苏大学附属人民医院心内科,江苏,镇江,212002
- 折叠
摘要
Abstract
Objective: To construct miR-133a eukaryotic expression vector and transfect it into H9C2 cell. Methods: Design and synthetize the PCR templa-primer. Ligate the pre-miR-133a with linearized PgenesIL-1. 1 by T4 DNA Ligase. The recombinants were identified by endonuclease digestion and sequenced. Transient transfect the perfect recombinants into H 9C2 cells by LipofectamineTM 2000 Reagent. Finally, detect transfection efficiency by flow cytometer and invert fluorescence microscope . Results: The miR-133a eukaryotic expression vectors were consistent with the design and the transfection efficiency of H9C2 cells was 70％. Conclusion: miR-133a eukaryotic expression vector was successfully constructed and transfected into H9C2 cell.关键词
miR-133a/真核表达载体/凋亡/转染分类
医药卫生引用本文复制引用
张洪涛,张赢予,周艳芳,王好,郑枫,张国辉..miR-133a真核表达载体的构建及转染大鼠心肌H9C2细胞[J].江苏大学学报（医学版）,2011,21(1):26-28,49,封3,5.
