中国组织工程研究与临床康复2011，Vol.15Issue(6)：1123-1126,4.DOI:10.3969/j.issn.1673-8225.2011.06.039
干细胞微环境的体外模拟
Simulation of stem cell microenvironment in vitro
摘要
Abstract
BACKGROUND: Extracellular matrix (ECM) is an important component of the cellular niche in a tissue, supplying critical biochemical and physical signals to sustain cellular functions.OBJECTIVE: To review the research progress of in vitro simulation of bone marrow stem cell microenvironment, and to introduce reconstruction of ECM in vitro.METHODS: The database of Pubmed (1999-01/2010-10) and Embase (1999-01/2010-10) was utilized to search the related articles about stem cell microenvironment. Key words were "mesenchymal stem cells, extracellular matrix, niche, growth factor mod ulation, culture in vitro". There were 321 articles after the initial su rvey. A total of 23 articles related to stem cell microenvironment were selected according to inclusion criteria.RESULTS AND CONCLUSION: The established bone marrow-derived ECM culture system retains mesenchymal stem cell properties and may facilitate efforts to pu rify these cells and to produce a large number of relatively homogenous mesenchymal stem cells. Establishment of a unique scaffold built by 3D synthetic materials coated with tissue-specific ECM proteins and deposited with an appropriate combination of growth factors will facilitate the control of the differentiation direction of mesenchymal stem cells.关键词
微环境/间充质干细胞/细胞外基质/生长因子调控/体外培养分类
医药卫生引用本文复制引用
邱云,郑青,萧树东,汪铮..干细胞微环境的体外模拟[J].中国组织工程研究与临床康复,2011,15(6):1123-1126,4.基金项目
国家自然科学基金(30971468). （30971468）
