中国组织工程研究与临床康复2011，Vol.15Issue(7)：1179-1185,7.DOI:10.3969/j.issn.1673-8225.2011.07.009
DA至Lewis大鼠肝移植急性排斥反应模型的建立:技术改良分析
Construction of a rat DA to Lewis orthotopic liver transplantation model:Technical improvement
摘要
Abstract
BACKGROUND:The acute rejection model of rat liver transplantation is the basic for studying the immunologic tolerance of animals,and the accepted matching pattern was DA to Lewis rats,DA to BN rats,and BN to Lewis rats.The above mentioned matching methods are few in China due to insufficient rat species and immature operation technology.OBJECTIVE:To establish rat (DA to Lewis) orthotopic liver transplantation model and to explore the skill and experience for establishing this model.METHODS:A total of 60 rats were established orthotopic liver transplantation models using Kamada two-cuff technique. Male DA rats served as donors, and male Lewis rats were used as recipients. Therapeutic amount of tacrolimus was administrated to recipients during 1 day before operation and 1 week after operation, which was continuously half dose decreasing every day began from eighth day post-operation and stopped administration by thirteenth day post-operation. The operation time was recorded, and the survival status, success rate and survival time was observed. All rats were sacrificed at 7, 14, 21 and 28 days after operation. The gross and histopathologic changes of liver tissues were observed. And rejection activity index of liver tissues was scored.RESULTS AND CONCLUSION:The cold ischemia time of donor liver was 30-60 minutes, the donor operation time, graft truing time, recipient operation time, and anhepatic period were (18.5±4.0) minutes, (7±3) minutes, (35.0±7.3) minutes, (13.0±3.0)minutes, respectively. The operation success rate and one week survival rate were 98％ and 91 .6％, respectively. Acute rejection occurred rapidly 2 weeks after liver transplantation due to tacrolimus discontinuation. Recipient's dead time distributed from 28 days after the operation and mean survival time were (20.85±0.71)days, and the median survival time was 21 days respectively. Based on improvements in Kamada two-cuff technique and short-term tacrolimus application on perioperative period, DA to Lewis rat acute rejection orthotopic liver transplantation models were stably established.关键词
急性排斥反应/DA大鼠/Lewis大鼠/原位肝移植术/动物模型/他克莫司分类
医药卫生引用本文复制引用
李铸,李立,冉江华,张升宁,刘静,刘滇生,李来邦,陈娟..DA至Lewis大鼠肝移植急性排斥反应模型的建立:技术改良分析[J].中国组织工程研究与临床康复,2011,15(7):1179-1185,7.基金项目
云南省科技厅基金项目(2007CA-007)的支持,项目名称:多脏器联合移植重大关键技术的研究及应用示范. （2007CA-007）
