中国免疫学杂志2011，Vol.27Issue(3)：246-250,5.DOI:10.3969/j.issn.1000-484X.2011.03.013
IL-23、IL-27对RSV重组蛋白疫苗免疫原性的影响
Effect of IL-23 or IL-27 on the immunogenity of the respiratory syncytial virus recombinant protein vaccine
摘要
Abstract
Objective :Mice were co-immunized with the recombinant vaccines G1F/M2 of respiratory syncytial virus (RSV ) and the adjuvant plasmids either pcDNA3.1-IL-23 or pcDNA3 .1-IL-27 to observe the effect of IL-23 or IL-27 on the immunogenity of the candidate vaccine .Methods :BALB/c mice were immunized with the recombinant vaccine encoding G1F/M2 combined with the plasmids either pcDNA3 .1-IL-23 or pcDNA3 .1-IL-27 and/or AI(OH )3 as adjuvant .Mice were killed at 10 days after the last immunization .Anti-G1F/M2 IgG , IgC1 ,IgG2a in the sera were detected by ELISA .The lymphocyte phenotype of CD4+ or CD8+ was analyzed by FCM ,and their splenocytes were tested for CTL activity by LDH-release assay .Results :GIF/M2+Al(OH )3+IL-23 and G1F/M2+Al(OH )3+ IL-27 both induced high titers of IgG ,IgG1 and IgG2a antihodies .The antibody levels of the immunized mice with G 1F/M2+Al(OH )3+IL-23 or GIF/M2+Al (OH )3+ IL-27 groups were significantly higher than those of G1F/M2+Al(OH )3 ,G1F/M2+ IL-23 , or G1F/M2+ IL-27 alone .The levels of CD4+ and CD8+T cells in the groups of G1F/M2+IL-23 and G1F/M2+Al(OH )3+ IL-23 were significantly higher than in other groups .IL-23 or IL-27 alone did not enhanced the CTL activity induced by G1F/M2 ,but immunization with G1F/M2+Al (OH )3 + IL-23 or G1F/M2+ Al(OH )3+ IL-27 induced significantly higher level of CTL than G1F/M2+Al (OH )3 ,G1F/M2+ IL-23 , or G1F/M2+ IL-27 .Conclusion : These results indicates that combining IL-23 and Al(OH )3 ,or IL-27 and AI(OH )3 could enhance the immunogenicity of candidate RSV vaccine G1F/M2 significantly .关键词
IL-23/IL-27/呼吸道合胞病毒(RSV)/G1F/M2/Al(OH)3/佐剂分类
医药卫生引用本文复制引用
杨晨涛,魏林,曾瑞红..IL-23、IL-27对RSV重组蛋白疫苗免疫原性的影响[J].中国免疫学杂志,2011,27(3):246-250,5.基金项目
本文为国家自然科学基金(30972801)、教育部科技重点项目(209009) （30972801）
