中国免疫学杂志2011，Vol.27Issue(3)：265-268,273,5.DOI:10.3969/j.issn.1000-484X.2011.03.017
系统性红斑狼疮患者血清多肽的MALDI-TOF MS分析研究
A proteomic study of serum peptidome patterns of human systemic lupus erythematosus based on MALDI-TOF mass spectrometry analysis
摘要
Abstract
Objective :To find differentially expressed disease-related and condition-specific peptides in sera from patients with SLE by utilization of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALD1-TOF-MS) ,and to develop and validate the peptide classification model for diagnosis of SLE.Methods :SLE patients were divided into two groups ,active SLE and stable SLE ,and healthy donors were as normal control.We performed magnetic beads-based weak cation exchange for sample processing and MALDI-TOF-MS for peptide profiling.ClinProt software 2.1 (Bruker Daltonics Company ) was used for data analysis and genetic algorithm was modeled for class prediction.Results :A series of significant short peptides were successfully detected and identified.Classification models were developed ,and achieved high capability of prediction and cross-validation.Blinded verification of the classification model showed 91.7％ sensitivity in active-SLE,83.3％ sensitivity in stable-SLE and 86.7％ specificity in normal controls.Conclusion :A preliminary work is done for a better understanding of the pathogenesis of SLE and improving diagnosis of SLE from an integrated perspective of peptide mass fingerprinting.关键词
系统性红斑狼疮/蛋白质组学/质谱/分析分类
医药卫生引用本文复制引用
文锦丽,戴勇,黄礼玲,胡成效,王琳纤..系统性红斑狼疮患者血清多肽的MALDI-TOF MS分析研究[J].中国免疫学杂志,2011,27(3):265-268,273,5.基金项目
本文为深圳市科技计划项目(200902012) （200902012）
